PXD067326

PXD067326 is an original dataset announced via ProteomeXchange.

Title	L-type Pyocins Inhibit the BAM Complex to Kill Without Cell Entry
Description	The Gram-negative bacterium Pseudomonas aeruginosa can be an opportunistic human pathogen that is naturally resistant to many antibiotics and often develops further multidrug resistance. The protein antibiotic group of L-type pyocins can clear lethal MDR P. aeruginosa infections in mouse lungs but their mode of action is unknown. In this study, we show with cryo-EM that L-type pyocins inhibit the P. aeruginosa BAM complex in a multi-step mechanism. In the pre-inhibition state, L-type pyocins bind to the extracellular loop 6 of BamA and in the full inhibition state, the C-terminal extension blocks the lateral gate from accepting nascent outer membrane proteins. With cryo-ET, we show that BAM inhibition is cytotoxic by the excessive formation of vesicles leading to outer membrane disruption. To determine the cellular consequences of L-type pyocin intoxication, we combine transposon-directed insertion site sequencing (TRADIS), transcriptomics, proteomics, finding that L-type pyocins inhibit the BAM complex, resulting from a loss of integrity of the outer membrane and ultimately cell death.
HostingRepository	PRIDE
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-03_17:46:15.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Imogen Samuels
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1H2O; NCBI TaxID: NEWT:1147787;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 16:27:39	ID requested
⏵ 1	2026-06-03 17:46:16	announced

Dataset with its publication pending

submitter keyword: BAM pyocin OMP

Rhys William Grinter
contact affiliation	Lab Head, Department of Biochemistry and Pharmacology, The University of Melbourne
contact email	rhys.grinter@unimelb.edu.au
lab head
Imogen Samuels
contact affiliation	University of Melbourne
contact email	igsamuels@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067326

PRIDE project URI

• PRIDE
  1. PXD067326
     1. Label: PRIDE project
     2. Name: L-type Pyocins Inhibit the BAM Complex to Kill Without Cell Entry