PXD067301

PXD067301 is an original dataset announced via ProteomeXchange.

Title	Host serum-derived flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense
Description	Flaviviruses pose growing global health threats and are transmitted by Aedes mosquitoes. Yet, how these viruses overcome early mosquito immunity remains unclear. Here, we reveal a cross-host immune evasion strategy in which the flavivirus NS1 protein, abundantly secreted into infected human serum, is taken up by mosquitoes during blood feeding and rapidly internalized into midgut epithelial cells—prior to viral replication. NS1 hijacks the mosquito spliceosome-associated factor Prp19 to promote miR-275-5p maturation, which suppresses the caspase gene CASPS19, blocking apoptosis and enabling early viral replication. Engineered mosquitoes expressing a miR-275 sponge show enhanced resistance to dengue and Zika viruses. These findings uncover a previously unrecognized mechanism whereby a vertebrate-derived viral protein reprograms vector gene regulation, establishing a new paradigm of cross-species immune manipulation by flaviviruses.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_06:37:41.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yifei Li
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: NEWT:7159;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 08:10:08	ID requested
⏵ 1	2026-08-18 06:37:42	announced

10.1038/s41467-026-72631-1;

Li Y, Cui C, Ding J, Wang Y, Yun J, Li F, Wang S, Host serum-circulating flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: midgut,Aedes aegypti, LC-MS/MS

Sibao Wang
contact affiliation	CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
contact email	sbwang@cemps.ac.cn
lab head
yifei Li
contact affiliation	CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
contact email	liyifei@cemps.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD067301

PRIDE project URI

• PRIDE
  1. PXD067301
     1. Label: PRIDE project
     2. Name: Host serum-derived flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense