PXD067297

PXD067297 is an original dataset announced via ProteomeXchange.

Title	Atypical features of the disordered N-terminal region of a bacterial outer membrane copper transporter.
Description	Metals like copper (Cu), zinc, and nickel exhibit dual nature, necessitating a tight regulation of their cellular homeostasis to meet physiological demands while preventing toxicity. In bacteria, metal homeostasis involves inner membrane P-type ATPases and ABC transporters, envelope-spanning tripartite efflux pumps, and outer membrane pore-forming proteins. Four decades ago, the outer membrane β-barrel protein PcoB was shown to provide an additional layer of copper resistance in an E. coli strain isolated from the gut of swine fed with Cu supplements. Interestingly, most PcoB homologs contain a poorly conserved disordered N-terminal domain (NTD) rich in histidine and methionine residues, which are commonly associated with copper coordination in cuproproteins. This suggests a potential role for the NTD in PcoB-mediated copper efflux. We previously demonstrated that the free-living bacterium Caulobacter vibroides primarily relies on PcoB for copper homeostasis. Here, we show that the NTD of C. vibroides PcoB is critical for PcoB function and stability, tolerating the swapping with the poorly conserved E. coli PcoB NTD and significant truncations. Unexpectedly, the predicted signal peptide was dispensable, challenging traditional concepts of protein translocation mechanisms. Moreover, the PcoB NTD plays a surprising role in stabilizing the periplasmic multicopper oxidase PcoA, encoded within the same operon as PcoB, highlighting a new role for a disordered region.
HostingRepository	PRIDE
AnnounceDate	2025-09-05
AnnouncementXML	Submission_2025-09-05_02:36:09.726.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 06:34:11	ID requested
⏵ 1	2025-09-05 02:36:10	announced

10.1016/J.JBC.2025.110670;

submitter keyword: disordered region, efflux, stress response,Bacteria, Caulobacter, copper

Jean-Yves Matroule
contact affiliation	Research Unit in Biology of Microorganisms (URBM), Department of Biology, Namur Research Institute for Life Sciences (NARILIS), University of Namur, Namur, Belgium
contact email	jean-yves.matroule@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067297

PRIDE project URI

• PRIDE
  1. PXD067297
     1. Label: PRIDE project
     2. Name: Atypical features of the disordered N-terminal region of a bacterial outer membrane copper transporter.