PXD067290

PXD067290 is an original dataset announced via ProteomeXchange.

Title	Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and regulates formation of reovirus factories
Description	Early in infection, mammalian orthoreoviruses (reoviruses) build neo-organelles called viral factories (VFs). These structures incorporate fragments of endoplasmic reticulum (ER) and serve as sites of viral genome replication and particle assembly. Two reovirus nonstructural proteins, σNS and µNS, remodel the ER to produce the vesicles and tubules that form the VF matrix. Using co-immunoprecipitation assays followed by mass spectrometry, we identified annexin A2 (ANXA2), which binds actin and cellular membranes, as a host factor that interacts with reovirus nonstructural proteins. In the absence of ANXA2, VF formation was accelerated in the early phases of reovirus infection, and yields of reovirus were increased. Moreover, organization of the ER network and structure of the actin cytoskeleton were altered in the absence of ANXA2, suggesting that ANXA2 binding to actin is required to maintain ER network morphology. These findings provide evidence that interactions of reovirus nonstructural proteins directly or indirectly with ANXA2 are required for ER remodeling and formation of the membranous fragments that serve as the matrix to build reovirus factories
HostingRepository	PRIDE
AnnounceDate	2025-10-29
AnnouncementXML	Submission_2025-10-29_05:47:47.560.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067290
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Reovirus sp.; NCBI TaxID: NEWT:10891;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 02:14:32	ID requested
⏵ 1	2025-10-29 05:47:48	announced

10.6019/PXD067290;

10.1128/JVI.01389-25;

submitter keyword: reovirus, σNS, endoplasmic reticulum, actin, viral factories,Annexin A2, µNS

Cristina Risco
contact affiliation	Cell Structure Laboratory, National Center for Biotechnology (CNB-CSIC)
contact email	crisco@cnb.csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067290
     1. Label: PRIDE project
     2. Name: Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and regulates formation of reovirus factories