PXD067287

PXD067287 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry Analysis of C. elegans ISW-1::GFP
Description	The germ cell nuclear extracts of ISW-1::GFP were incubated with GFP Nanoab Agarose Beads (GNA-25-500, NuoyiBio) for 6 hours at 4°C. The mixture was then washed five times with lysis buffer (20 mM Tris-HCl pH 7.6, 300 mM NaCl, 1.5 mM MgCl2, 20 mM KCl, 1% NP-40, 1 mM DTT, and protease inhibitors). Immunoprecipitated proteins were eluted by boiling in 1% SDS and 25 mM Tris buffer (pH 7.4) for 5 minutes. The eluted proteins were precipitated with 20% trichloroacetic acid (TCA) at 4°C for 1 hour, followed by centrifugation at 14,000 rpm for 10 minutes at 4°C. The precipitate was then washed once with 10% TCA and twice with cold acetone (14,000 rpm for 10 minutes at 4°C). The final protein pellet was collected and digested with trypsin for mass spectrometry analysis.
HostingRepository	iProX
AnnounceDate	2025-08-13
AnnouncementXML	Submission_2025-11-30_17:38:12.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruoxi Wang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-12 23:21:00	ID requested
⏵ 1	2025-11-30 17:38:13	announced

Dataset with its publication pending

submitter keyword: GFP

Jinmin Gao
contact affiliation	Shandong Normal University
contact email	jinmingao@sdnu.edu.cn
lab head
Ruoxi Wang
contact affiliation	Shandong Normal University
contact email	wangruoxi@sdnu.edu.cn
dataset submitter

iProX dataset URI