PXD067282

PXD067282 is an original dataset announced via ProteomeXchange.

Title	Human 4D-label free quantitative proteomics of migrasomes from DOK, DOK-BD and DOK-4NQO cells
Description	Oral leukoplakia (OLK), a recognized oral potentially malignant disorder, demonstrates significant susceptibility to malignant transformation into oral squamous cell carcinoma (OSCC). This study aimed to investigate the involvement of migrasomes, novel intercellular communication organelles, in the carcinogenesis of OLK. Migrasomes were observed through electron microscopy and multiplex immunofluorescence in OLK and OSCC tissues, and the purified migrasomes were characterized by wheat germ agglutinin staining, long-term imaging, Western blot, and electron microscopy. Single-cell RNA sequencing analysis was conducted to evaluate the effect of migrasomes on the microenvironment. The combined analysis of the quantitative proteome of migrasomes and the transcriptome of recipient cells was used to investigate the involvement of migrasomes in the carcinogenesis of OLK. Xenograft tumors and a mouse model of tongue leukoplakia helped verify migrasomes’ participation in carcinogenesis. Migrasomes were found in both human OLK and OSCC tissues. Notably, migrasomes isolated from dysplastic oral keratinocyte DOK cells, DOK-transformed cells, and OSCC cell lines exhibited characteristic morphological features and distinct molecular signatures. A significant correlation existed between migrasome score and the infiltration of immune cells. Moreover, the protein cargoes within migrasomes promoted leukoplakia carcinogenesis by inducing an immunosuppressive microenvironment. This study reveals that migrasomes drive leukoplakia carcinogenesis. It not only deepens the understanding of the interaction between dysplastic keratinocytes and immune cells during this process but also provides potential diagnostic biomarkers or targets for the treatment of the precancer stage of OSCC.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:15:28.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoyu Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-12 20:41:24	ID requested
⏵ 1	2026-06-07 16:15:29	announced

10.1038/s41467-026-70824-2;

Jiang MJ, Zhou HY, Bai YT, Chen XJ, Zhou G, macrophages to drive oral leukoplakia carcinogenesis. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: Human, OLK, Migrasomes, OSCC

Gang Zhou
contact affiliation	Wuhan university
contact email	zhougang@whu.edu.cn
lab head
Haoyu Zhou
contact affiliation	Wuhan university
contact email	zhouhy@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067282

PRIDE project URI

• PRIDE
  1. PXD067282
     1. Label: PRIDE project
     2. Name: Human 4D-label free quantitative proteomics of migrasomes from DOK, DOK-BD and DOK-4NQO cells