PXD067269 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiles of the Antarctic diatom Fragilariopsis cylindrus under varying iron and manganese conditions |
| Description | Fragilariopsis cylindrus is an abundant diatom species in the Southern Ocean, where low iron and manganese availability constrain microbial growth and biogeochemical cycles. The molecular mechanisms used by polar diatoms including F. cylindrus to cope with trace metal limitations remain largely unexplored. Here we present phenotypic characterizations and high resolution proteomics profiles of F. cylindrus grown under controlled iron (low, medium, high) and manganese (low, high) conditions that reflect those observed in the Southern Ocean. Using data-independent acquisition mass spectrometry, we quantified over 8,000 unique proteins with high reproducibility. We captured diverse metabolic responses related to photosynthesis, elemental transport and intracellular trafficking, and protein synthesis. We show that several canonical iron stress response proteins (e.g., phytotransferrin) are consistently identified under low iron conditions, and identify additional useful iron and manganese stress biomarkers that could be explored in field measurements. Our data also support the notion that one flavodoxin copy in F. cylindrus is iron responsive and one is not. This dataset is a valuable resource for understanding trace metal physiology in polar diatoms, and provides a way for connecting molecular responses with expressed phenotypes and biogeochemical activity in the ocean. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_23:31:42.815.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Loay Jabre |
| SpeciesList | scientific name: Fragilariopsis cylindrus CCMP1102; NCBI TaxID: NEWT:635003; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-12 10:37:43 | ID requested | |
| ⏵ 1 | 2026-05-24 23:31:43 | announced | |
Publication List
| 10.1002/pmic.70109; |
| Jabre LJ, Rowland E, McCain JSP, Bertrand EM, Deep Proteomic Profiles of the Antarctic Diatom Fragilariopsis Cylindrus Under Varying Iron and Manganese Conditions. Proteomics, 26(5):112-119(2026) [pubmed] |
Keyword List
| submitter keyword: Diatoms |
| phytoplankton, trace metals |
| Southern Ocean |
| DIA |
Contact List
| Dr. Erin Bertrand |
|---|
| contact affiliation | Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada |
| contact email | erin.bertrand@dal.ca |
| lab head | |
| Loay Jabre |
|---|
| contact affiliation | Dalhousie University |
| contact email | loay.jabre@dal.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067269
- Label: PRIDE project
- Name: Proteomic profiles of the Antarctic diatom Fragilariopsis cylindrus under varying iron and manganese conditions
