PXD067265

PXD067265 is an original dataset announced via ProteomeXchange.

Title	Astrocyte proteome from the rat substantia nigra. Study in animal model of fluorocitrate-induced astrocyte dysfunction and microglia activation.
Description	Dysfunction of astrocytic support for prolonged time can be harmful for nervous system. Inflammation and glia activation are among hallmarks of most neurodegenerative diseases. Therefore, astrocytes are considered as druggable targets for disease modifying strategies. Partial lesion of dopaminergic neurons is compensated functionally, but which mechanisms within each type of cells are responsible is unknown. The aim was to check how neuron vs astrocyte degeneration affected remaining astrocytes proteome and which mechanisms were regulated. In a rat model of 7-day infusion by osmotic minipumps of fluorocitrate (FC) into the substantia nigra (SN) (doi: 10.1007/s12035-017-0529-z; doi: 10.1111/jnc.14605; doi: 10.1016/j.mito.2018.12.002) astrocytes become dysfunctional and die inducing activation of remaining astrocytes and microglia. 6-OHDA injected into medial forebrain bundle caused selective degeneration of dopaminergic neurons in the SN. Controls were sham operated. Astrocytes were sorted out from dissociated SN tissue using FACS, and processed for mass spectrometry proteome analysis. Understanding the role of individual cell type in the diseased tissue cellular context is essential to understand disease pathomechanisms and identify better pharmacological targets.
HostingRepository	PRIDE
AnnounceDate	2026-02-11
AnnouncementXML	Submission_2026-02-11_01:45:03.732.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067265
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Przemyslaw Mielczarek
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-12 06:16:13	ID requested
⏵ 1	2026-02-11 01:45:04	announced

10.6019/PXD067265;

10.1016/J.DIB.2026.112473;

submitter keyword: proteomics, LC-MS, fluorocitrate, astrocyte, substantia nigra

Przemyslaw Mielczarek
contact affiliation	1) Department of Analytical Chemistry and Biochemistry, AGH University of Krakow, Krakow, Poland 2) Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland
contact email	mielcza@agh.edu.pl
lab head
Przemyslaw Mielczarek
contact affiliation	1) AGH University of Krakow, Krakow, Poland 2) Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland
contact email	przemyslaw.mielczarek@agh.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067265

PRIDE project URI

• PRIDE
  1. PXD067265
     1. Label: PRIDE project
     2. Name: Astrocyte proteome from the rat substantia nigra. Study in animal model of fluorocitrate-induced astrocyte dysfunction and microglia activation.