PXD067233 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optimising the induction of inflammation within preterm infant-derived intestinal epithelial organoids |
| Description | Preterm infants born <32 weeks gestation have abnormal microbial colonisation and dysregulated inflammation within the gut. Preterm infant-derived intestinal organoids (PIOs) represent a valuable model for investigating gut microbiome-host interactions and inflammatory responses. We optimised an inflammation model in PIO monolayers incubated within an anaerobic co-culture system that recreates the physiological oxygen gradient of the intestinal epithelium. We trialled multiple stimuli, including live and heat-killed pathobiont consortia, lipopolysaccharide (LPS) and flagellin. We found that a combination of apical LPS and basolateral flagellin, incubated for 3 hours, elicited the most robust response. This was characterised by enhanced pro-inflammatory cytokine secretion, the potential for chemokine-driven immune recruitment, TNFα and IL17C pathway signalling, shifts from NF-κB to AP-1-mediated responses, and signs of tissue remodelling. This provides a framework for appropriate study design to disentangle the impacts of microbiome-host interactions in health and disease using intestinal organoids. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-17 |
| AnnouncementXML | Submission_2026-01-16_19:59:06.832.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Matthias Trost |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-11 15:42:36 | ID requested | |
| ⏵ 1 | 2026-01-16 19:59:07 | announced | |
Publication List
Keyword List
| submitter keyword: preterm models, gut inflammation, human, host-microbiome interactions, intestinal organoid,preterm infant, bacteria |
Contact List
| Matthias Trost |
|---|
| contact affiliation | Newcastle University Biosciences Institute Cookson Building Newcastle University NE2 4HH United Kingdom |
| contact email | matthias.trost@ncl.ac.uk |
| lab head | |
| Matthias Trost |
|---|
| contact affiliation | Newcastle University |
| contact email | matthias.trost@ncl.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067233 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067233
- Label: PRIDE project
- Name: Optimising the induction of inflammation within preterm infant-derived intestinal epithelial organoids
