PXD067228

PXD067228 is an original dataset announced via ProteomeXchange.

Title	Circulating extracellular vesicle protein biomarker associated with early cancer lesions for early detection of high-grade serous ovarian cancer
Description	Small extracellular vesicles (sEVs), lipid-bilayer delimited particles (50-200 nm) released by cells, are considered a new class of liquid biopsy biomarkers for stealth cancers, e.g., HGSOC. HGSOC originates from the fallopian tube (FT) and progresses from p53 signatures to a precursor lesion called serous tubal intraepithelial carcinoma (STIC). We hypothesize that sEVs play a role in ovarian pathogenesis, carry cargo reflective of the site of origin, and are biomarkers for early detection. To advance this idea, we established a case-control study cohort of archival plasma samples from 30 HGSOC patients (10 early-stage and 20 late-stage) and 40 healthy controls. sEVs were isolated using size exclusion chromatography, and proteomic profiles were established using mass spectrometry (LC-MS/MS). 1,078 EV proteins (exo-proteins) were identified across all samples, with 52 upregulated exo-proteins in early-stage HGSOC, and 59 upregulated exo-proteins in late-stage HGSOC (logFC>1, p<0.05). Upregulated exo-proteins were prioritized based on FT origin and tissue expression in STIC lesions. Seven candidate exo-proteins were validated by immunohistochemistry in FT with STIC lesions and HGSOC tissues and by western blot in FT/HGSOC cell-derived EVs. In summary, our EV proteomics using early-stage HGSOC clinical samples uncovered exo-biomarkers for early detection; potentially while still confined to the FT.
HostingRepository	PRIDE
AnnounceDate	2026-01-09
AnnouncementXML	Submission_2026-01-08_17:41:35.430.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067228
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dennis Province
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	O4'-phospho-L-tyrosine; O-phospho-L-threonine; acetylated residue; monohydroxylated residue; O-phospho-L-serine; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 15:01:10	ID requested
⏵ 1	2026-01-08 17:41:35	announced

10.6019/PXD067228;

10.1016/J.MCPRO.2026.101508;

submitter keyword: Liquid Biopsy Biomarker, Early Cancer Detection,Extracellular vesicles, Proteomics

Andrew K. Godwin
contact affiliation	University of Kansas Medical Center
contact email	agodwin@kumc.edu
lab head
Dennis Province
contact affiliation	UAMS
contact email	dprovince@uams.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067228
     1. Label: PRIDE project
     2. Name: Circulating extracellular vesicle protein biomarker associated with early cancer lesions for early detection of high-grade serous ovarian cancer