PXD067219

PXD067219 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of LysoIP from HeLa untagged, Control and ASAH1-/-
Description	This quantitative proteomic analysis of lysosome-enriched fractions isolated by immunoprecipitation (LysoIP) from HeLa cells expressing TMEM192-3xHA, untagged controls, and ASAH1 knockout lines. Triplicate biological replicates were processed using TMTpro 18-plex labeling and high-resolution Orbitrap mass spectrometry with FAIMS. Data were searched against the human proteome with stringent FDR filtering and reporter ion quantification, enabling comparative profiling of lysosomal protein composition and changes associated with ASAH1 deficiency.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_04:36:40.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Kraus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 14:46:27	ID requested
⏵ 1	2025-08-12 04:36:40	announced

Dataset with its publication pending

submitter keyword: lysoIP, PD, iN, iDA, lysosome dysfunction, neurodegeneration,TMTpro

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA, USA
contact email	wade_harper@hms.harvard.edu
lab head
Felix Kraus
contact affiliation	Harvard Medical Sschool
contact email	felix_kraus@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD067219

PRIDE project URI

• PRIDE
  1. PXD067219
     1. Label: PRIDE project
     2. Name: Proteomic analysis of LysoIP from HeLa untagged, Control and ASAH1-/-