PXD067201

PXD067201 is an original dataset announced via ProteomeXchange.

Title	Whole cell proteomics of iNeurons and iDA neurons at day 23 from Control, ASAH1-/- and SMPD1-/-
Description	This experiment describes a quantitative proteomic analysis of whole-cell lysates from induced neurons (iN) and induced dopaminergic neurons (iDA) differentiated from human embryonic stem cells of three genotypes: control, ASAH1 knockout, and SMPD1 knockout. Triplicate biological samples were prepared and analyzed using TMTpro 18-plex labeling coupled to high-resolution Orbitrap mass spectrometry with FAIMS. Data were processed with target-decoy database searching, stringent FDR control, and reporter ion–based quantification. The resulting dataset enables comparative profiling of proteomic alterations associated with lysosomal dysfunction and neurodegeneration-relevant pathways in distinct neuronal subtypes.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_04:39:47.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Kraus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 08:58:09	ID requested
⏵ 1	2025-08-12 04:39:47	announced

Dataset with its publication pending

submitter keyword: PD, iN, iDA, lysosome dysfunction, neurodegeneration,TMTpro

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA, USA
contact email	wade_harper@hms.harvard.edu
lab head
Felix Kraus
contact affiliation	Harvard Medical Sschool
contact email	felix_kraus@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD067201

PRIDE project URI

• PRIDE
  1. PXD067201
     1. Label: PRIDE project
     2. Name: Whole cell proteomics of iNeurons and iDA neurons at day 23 from Control, ASAH1-/- and SMPD1-/-