PXD067198

PXD067198 is an original dataset announced via ProteomeXchange.

Title	Lectin-Fc(IgG2a) fusion proteins binding to the cell wall of C. albicans cause structural, metabolic, oxidative, and other pleiotropic effects
Description	Candida albicans, an opportunist fungal pathogen ranked top among those causing hospital-acquired infections, exhibits diverse virulence factors aiding its survival within the human body. The rise of multidrug resistance (MDR) in C. albicans requires urgent development of new alternative antifungal therapies. Through genetic engineering gene fusion technologies, our group developed Lectin- Fc(IgG2a) proteins (WGA-Fc(IgG) and Dectin-1-Fc(IgG)) exhibiting antifungal effects against various endemic mycosis and consisting of promising options to combat fungal infections. This study explores the direct impact of Lectin-Fc treatments on C. albicans, shedding light on potential mechanisms disrupting fungal survival and growth. Our results revealed Lectin-Fc(IgG2a) proteins' ability to reduce key polysaccharides on the fungal cell wall. Co-incubations also resulted in oxidative stress in the fungus and alterations in the yeast micronutrient content and lipid reserves. Furthermore, Lectin-Fc(IgG2a) impacted fungal secretion and impeded the release of extracellular vesicles (EVs). The proteomic analysis demonstrated differential protein expression in both the whole C. albicans yeast and EVs post-Lectin-Fc(IgG2a). Notably, these antibody-like structures are slowly degraded by secreted aspartyl proteases (Saps) in vitro, as sap mutants are more prone to growth inhibition. Finally, Lectin-Fc(IgG2a) opsonization enhanced the activation of dendritic cells, contributing to fungal killing. These findings affirm Lectin-Fc(IgG2a) proteins as viable alternatives for therapeutic interventions against fungal infections. Beyond their antibodies-like functions, they induce fungal morphological alterations, and oxidative and metabolic stress, increasing the susceptibility of their cell wall to host immune recognition and elimination.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-06_07:15:02.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Guimaraes
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: NEWT:5476;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 07:54:26	ID requested
⏵ 1	2026-04-06 07:15:03	announced

Dataset with its publication pending

submitter keyword: Candida albicans. Lectin-Fc, antifungal effects

Allan Jefferson Guimaraes
contact affiliation	Fluminense Federal Unibersity
contact email	allanguimaraes@id.uff.br
lab head
Allan Guimaraes
contact affiliation	Federal Flumunense University
contact email	allanguimaraes@id.uff.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067198
     1. Label: PRIDE project
     2. Name: Lectin-Fc(IgG2a) fusion proteins binding to the cell wall of C. albicans cause structural, metabolic, oxidative, and other pleiotropic effects