PXD067161

PXD067161 is an original dataset announced via ProteomeXchange.

Title	APOE genotype shapes lipid droplet composition and dynamics in human astrocytes
Description	Lipid droplets are dynamic organelles involved in cellular functions ranging from lipid storage regulation to immune modulation. The proteins that localize to the lipid droplet membrane play a critical role in determining these functions. Recent research has highlighted the involvement of glial lipid droplets in the pathology and progression of late-onset Alzheimer’s disease. APOE, a gene encoding a lipid-binding and transport protein, is a major genetic determinant of Alzheimer’s disease risk, with different variants conferring risk or protection. In this study, we characterized the human astrocytic lipid droplet proteome and found that each APOE variant–risk, benign, and protective–produces a distinct proteomic profile in astrocytes. These variant-specific proteomes influence lipid droplet biology and broader aspects of cellular physiology. Moreover, we discovered that changes in the lipid droplet proteome directly affect the turnover and dynamics of lipid droplets in astrocytes across different APOE genotypes. Together, these findings provide new insights into how APOE variants reshape astrocyte biology and identify lipid droplets as a modifiable aspect of Alzheimer’s disease pathology.
HostingRepository	PRIDE
AnnounceDate	2026-07-01
AnnouncementXML	Submission_2026-07-01_10:24:17.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Andy Qi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-10 14:04:56	ID requested
⏵ 1	2026-07-01 10:24:18	announced

10.1101/2025.08.19.669163;

Cun, í, -L, ó, pez C, Root JT, Hao Y, Kowal I, Blomberg N, Ghirlando R, Yang LG, Koppes-den Hertog SJ, Cookson MR, van der Kant R, Giera M, Qi YA, Narayan PS, genotypes differentially remodel the astrocytic lipid droplet-associated proteome to shape lipid droplet dynamics. bioRxiv, ():(2025) [pubmed]

submitter keyword: lipid proteomics,APOE

astrocytes

Ying Hao
contact affiliation	Center for Alzheimer's and Related Dementias (CARD), NIA and NINDS, NIH, Bethesda, Maryland, USA.
contact email	ying.hao@nih.gov
lab head
Yue Andy Qi
contact affiliation	Center for Alzheimer’s Disease and Related Dementias (CARD)/NIH
contact email	andy.qi@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD067161

PRIDE project URI

• PRIDE
  1. PXD067161
     1. Label: PRIDE project
     2. Name: APOE genotype shapes lipid droplet composition and dynamics in human astrocytes