PXD067158

PXD067158 is an original dataset announced via ProteomeXchange.

Title	Differentially palmitoylated proteins in mitochondria from wild-type and ABHD10-knockout mice LC-MSMS
Description	Palmitoylation is the only fully reversible post-translational lipid modification that impacts 10-20% of the human proteome, but its role during spermatogenesis remains enigmatic. In this study, through generating HA-tagged Abhd10 knock-in mice, Abhd10-null mice, and combining super-resolution fluorescence imaging and electron microscopy, we identified that the S-depalmitoylase ABHD10 is a mitochondrial matrix protein, specifically expressed in testis and is essential for male fertility. Abhd10 knockout mice manifest severe sperm motility defects accompanied by malformed mitochondrial sheaths of sperm. Strikingly, mitochondrial proteomic revealed that ABHD10 deficiency primarily disrupts respiratory chain complexes. Further, mass spectrometry-based mitochondrial acyl - biotin exchange (ABE) assays identified that ABHD10 catalyzes S-depalmitoylation of proteins involved in aerobic respiration and respiratory electron transport, particularly PDHX—key regulators of oxidative phosphorylation. In addition, ABHD10 could form complex with PDHX, thereby catalyzing its S-depalmitoylation. Overall, this work identifies S-depalmitoylase as a key determinant of male fertility and unveils the facilitating role of ABHD10-mediated S-depalmitoylation in oxidative phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-20_03:52:47.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ziqi Yu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-10 03:40:05	ID requested
⏵ 1	2025-10-20 03:52:48	announced

10.1038/S41467-025-65300-2;

submitter keyword: mouse testis mitochondria palmitoylated

Fei Sun
contact affiliation	Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China
contact email	sunfeisrrsh@zju.edu.cn
lab head
ziqi Yu
contact affiliation	Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
contact email	mr.yuziqi@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067158
     1. Label: PRIDE project
     2. Name: Differentially palmitoylated proteins in mitochondria from wild-type and ABHD10-knockout mice LC-MSMS