PXD067144

PXD067144 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	VHL-AirID expressing HCC827-Luc cells treated with DMSO, VH032 or MS39
Description	HCC827-Luc cells stably expressing VHL-AirID were pretreated with MG132. The DMSO, VH032 or MS39 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_07:47:09.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; Acetyl; N6-biotinyl-L-lysine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-09 02:03:49	ID requested
⏵ 1	2025-09-03 07:47:10	announced

Publication List

Yamada K, Yamanaka S, Yamakoshi H, Kohyama A, Iwabuchi Y, Kosako H, Sawasaki T, In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID. Commun Biol, 8(1):1323(2025) [pubmed]

Yamada K, Yamanaka S, Yamakoshi H, Kohyama A, Iwabuchi Y, Kosako H, Sawasaki T, In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID. Commun Biol, 8(1):1323(2025) [pubmed]

Keyword List

submitter keyword: VHL, AirID, HCC827-Luc cells, VH032, MS39

submitter keyword: VHL, AirID, HCC827-Luc cells, VH032, MS39

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003993/