PXD067125

PXD067125 is an original dataset announced via ProteomeXchange.

Title	Investigating the Cytoprotective Mechanisms of the Tardigrade Damage Suppressor (Dsup) Protein in Human Cells Under Hypoxic Stress
Description	Introduction: Ischemia/reperfusion injury (IRI) is a common damage due to the restoration of blood flow after an ischemic insult. The pathogenesis of IRI is mainly linked to the production of ROS which sustain cell damage and ultimately promote cell death. The tardigrade damage suppressor protein (Dsup) is a DNA-binding protein that enables tardigrades to tolerate stress conditions including oxidative stress. Aim: We aim to investigate the ability of the tardigrade-derived Damage Suppressor (Dsup) protein to protect human cells from IRI, using an in vitro model of hypoxia and reoxygenation. Methods: The protective role of the tardigrade Dsup protein against ischemia-reperfusion injury was studied in transfected HEK293T cells. We analyzed cell viability, oxidative stress, and the expression of antioxidant proteins using functional assays, proteomics, and Western Blotting. Results: Dsup expression significantly enhanced cell survival following hypoxia-reoxygenation and markedly reduced intracellular ROS levels. Proteomic and Western Blot analyses revealed a significant upregulation of antioxidant enzymes in Dsup-expressing cells. Furthermore, Dsup modulated autophagy and key stress-related pathways, including the MAPK cascade. Dsup remained cytoplasmic under all conditions, and its expression was notably increased by hypoxic stimulus. Conclusion: This study demonstrate that Dsup protects human cells from ischemia-reperfusion injury by reducing oxidative stress and modulating key cytoprotective pathways. Our results establish Dsup as a promising candidate for future therapeutic applications against IRI, meriting further exploration in in vivo models.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:10:32.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enxhi Shaba
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-08 05:26:41	ID requested
⏵ 1	2025-11-23 16:10:33	announced

10.3390/ijms262110452;

Shaba E, Ricci C, Vantaggiato L, Paolocci MF, Regoli T, Miedviedieva K, Brunetti J, Ciccone V, Cecchin C, Donnini S, Marzocchi C, Landi C, Cantara S, Investigating the Cytoprotective Mechanisms of the Tardigrade Damage Suppressor (Dsup) Protein in Human Cells Under Hypoxic Stress. Int J Mol Sci, 26(21):(2025) [pubmed]

submitter keyword: ischemia, autophagy, reperfusion,Dsup, oxidative stress, proteomic

Prof. Claudia Landi
contact affiliation	Functional Proteomics Lab. Life Sciences Dept., University of Siena Siena, Italy
contact email	claudia.landi@unisi.it
lab head
Enxhi Shaba
contact affiliation	Functional Proteomics Lab, Life Sciences Department, University of Siena
contact email	enxhi.shaba@unisi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067125
     1. Label: PRIDE project
     2. Name: Investigating the Cytoprotective Mechanisms of the Tardigrade Damage Suppressor (Dsup) Protein in Human Cells Under Hypoxic Stress