PXD067097

PXD067097 is an original dataset announced via ProteomeXchange.

Title	Alternative preservation strategies of hearts donated after circulatory death: The effect of extended CIT before NMP
Description	Donation after circulatory death (DCD) offers a promising strategy to expand the donor pool by 20–30%. However, DCD heart grafts suffer from warm ischemia injury, making effective graft viability assessment before transplantation prudent. Current methods for assessing DCD hearts are impacted either by ethical concerns and regional limitations (thoracoabdominal normothermic regional perfusion - taNRP) or by the need for significant resources, expertise, and equipment, with limitations in functional assessment (direct procurement and reperfusion – DPP, via the Organ Care System (OCS) by TransMedics). This study explores an alternative approach to DCD heart preservation, using controlled hypothermia at 6-8 degrees celcius for up to 7 h, followed by reanimation using normothermic machine perfusion. No functional, biochemical or proteomic differences were observed between DCD cardiac grafts stored via controlled hypothermia for up to 7 h and DCD grafts reperfused immediately after procurement. These results suggest that controlled hypothermia may be a viable alternative to current DCD preservation methods, eliminating ethical concerns of taNRP and reducing logistical complexity and costs associated with DPP. These findings provide a foundation for a potential shift in DCD heart preservation practices, offering a more effective and cost-efficient approach to expand the donor pool and improve transplantation outcomes.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_18:47:53.436.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD067097
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marie Billaud
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 13:45:05	ID requested
⏵ 1	2026-08-23 18:47:54	announced

10.1161/jaha.125.044132;

Lopera Higuita M, Ajenu EO, Bolger-Chen M, Olverson G, Ghavimi SAA, Lee SY, Kasai T, Michaud W, Chijioke C, Ruidas B, Pitti A, Minie N, Catricala J, Vincent D, Li S, D'Alessandro D, Aikawa E, Singh SA, Billaud M, Osho AA, Tessier SN, Rabi SA, Alternative Preservation Strategies of Donation After Circulatory Death Hearts: Effect of Cold Ischemia Time Before Normothermic Machine Perfusion. J Am Heart Assoc, 15(12):e044132(2026) [pubmed]

10.6019/PXD067097;

submitter keyword: Hearts Transplantation

Swine

Cold Ischemia

Warm Ischemia

Tissue Donors

Hypothermia

Proteomics

Perfusion

S. Alireza Rabi
contact affiliation	Massachusetts General Hospital
contact email	seyedalireza.rabi@uhn.ca
lab head
Marie Billaud
contact affiliation	BRIGHAM AND WOMEN'S HOSPITAL
contact email	mbillaud@bwh.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067097
     1. Label: PRIDE project
     2. Name: Alternative preservation strategies of hearts donated after circulatory death: The effect of extended CIT before NMP