PXD067024

PXD067024 is an original dataset announced via ProteomeXchange.

Title	CD19+CD11c+T-bet+ B cells in myasthenia gravis: A potential biomarker
Description	Background: Myasthenia gravis (MG), an autoimmune disorder characterized by B cell-driven autoantibody production, exhibits heterogeneous B cell subsets dysregulation and incompletely defined signaling mechanisms. Methods: A cohort of 20 naïve MG patients positive for anti-acetylcholine receptor (AChR) antibodies and 15 healthy controls was analyzed. Peripheral blood mononuclear cells underwent proteomic profiling, flow cytometry (age-associated B cells (ABCs), plasma cells, T follicular helper cells, and regulatory B cells), and western blot validation of nuclear factor kappa-B (NF-κB)/cellular reticuloendotheliosis oncogene homolog (c-Rel) expression. Clinical severity was assessed using quantitative MG (QMG) scores. Statistical analyses included differential protein expression, pathway enrichment, and receiver operating characteristic (ROC) curve evaluation. Results: Proteomics revealed significant activation of the B cell receptor and NF-κB/c-Rel signaling pathways in MG patients, validated by upregulated NF-κB/c-Rel expression (P < 0.01). Flow cytometry demonstrated elevated ABCs (CD19⁺CD11c⁺T-bet⁺), plasma cells, and T follicular helper cells, alongside reduced regulatory B cells in MG (P < 0.001). The proportion of ABCs correlated positively with QMG scores (r = 0.5015, P = 0.024) but not with AChR antibody titers, suggesting antibody-independent mechanisms. ROC analysis identified moderate diagnostic utility of ABCs for moderate-to-severe MG (QMG scores 6; area under the curve = 0.68, 95 % confidence intervals: 0.42 – 0.94). Conclusions: This study establishes ABCs and NF-κB/c-Rel signaling as central contributors to AChR-MG immunopathology. Therefore, ABCs may serve as complementary biomarkers for clinical stratification.
HostingRepository	iProX
AnnounceDate	2025-08-07
AnnouncementXML	Submission_2025-08-12_19:36:59.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaru Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-06 20:28:52	ID requested
⏵ 1	2025-08-12 19:37:00	announced

Dataset with its publication pending

submitter keyword: myasthenia gravis, LC-MS/MS

Yaru Lu
contact affiliation	Henan Provincial People's Hospital
contact email	luyaru1994@163.com
lab head
Yaru Lu
contact affiliation	Henan Provincial People's Hospital
contact email	luyaru1994@163.com
dataset submitter

iProX dataset URI