PXD067019

PXD067019 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Fine-Tuning of Label-Free Single-Cell Proteomics Workflows
Description	This project aims to compare three distinct workflows using different sample collection/preparation plate supports (EVO96 oil-free, LF48 oil-based and LF48 oil-free, a streamlined automated sample resuspension and direct injection protocol). Then, digestion efficiency using a range of protein/enzyme ratios (1:1; 10:1; 20:1; 50:1) to maximize peptide recovery was evaluated. Finally, the chromatographic setup was refined to determine the best compromise between throughput and robustness.
HostingRepository	PRIDE
AnnounceDate	2025-12-13
AnnouncementXML	Submission_2025-12-12_20:22:09.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pauline Perdu-Alloy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Ultra 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-06 19:44:44	ID requested
⏵ 1	2025-12-12 20:22:09	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Label-FreeSCP

submitter keyword: Label-FreeSCP

Contact List

Christine CARAPITO
contact affiliation	CNRS, UMR7178, LSMBO, 67087 Strasbourg, France Université de Strasbourg, IPHC, 25 rue Becquerel, 67087 Strasbourg, France
contact email	ccarapito@unistra.fr
lab head
Pauline Perdu-Alloy
contact affiliation	LSMBO, IPHC, CNRS
contact email	perdualloy.pauline@yahoo.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD067019
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD067019

PRIDE project URI

Repository Record List

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