PXD067002 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Interactome analysis of DVL3 S/T cluster mutants using proximity labeling |
| Description | When WNT binds to its receptor Frizzled (FZD) to activate the signalling, Dishevelled (DVL), the central component of Wnt signalling, is phosphorylated by redundant Casein kinase 1 (CK1) isoforms ε or δ, to transduce the signal downstream. Here, we mutated key serine/threonine residues in the intrinsically disordered region (IDR) between the PDZ and DEP domains. Either to alanine to prevent phosphorylation, or to glutamic acid to mimic phosphorylation, or we deleted these residues altogether. We investigated the impact of these mutations on DVL’s interactome using proximity labeling with TurboID-tagged fusion proteins at N-terminus of DVL. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-07 |
| AnnouncementXML | Submission_2026-04-07_08:47:24.574.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Katerina Hanakova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-06 08:52:04 | ID requested | |
| ⏵ 1 | 2026-04-07 08:47:25 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Dishevelled, TurboID, proximity labeling, interactome |
Contact List
| Zbynek Zdrahal |
|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
| contact email | zdrahal@sci.muni.cz |
| lab head | |
| Katerina Hanakova |
|---|
| contact affiliation | CEITEC MU |
| contact email | katerina.hanak@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD067002 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067002
- Label: PRIDE project
- Name: Interactome analysis of DVL3 S/T cluster mutants using proximity labeling
