PXD066999

PXD066999 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Exosomes from Brucella abortus infected Macrophages Reveals Mechanisms of Immune Evasion and Host Modulation
Description	Brucella abortus is an intracellular pathogen capable of establishing chronic infections through sophisticated immune evasion mechanisms. Among these, exosomes derived from infected macrophages have emerged as key vehicles of pathogen–host communication. In this study, we performed a comparative proteomic analysis of exosomes secreted by RAW 264.7 macrophages infected with B. abortus at 8 and 24 hours post-infection using LC-MS/MS. Exosomes displayed dynamic and functionally distinct proteomic profiles depending on infection time. At 8 hpi, profiles showed enrichment of proteins related to biosynthesis, energy metabolism, and endoplasmic reticulum processing, along with reduced abundance of proteins associated with lysosomes and antigen presentation. At 24 hpi, profiles shifted toward mitochondrial and redox regulatory pathways, while suppression of immune-related processes persisted. Exclusive proteins were identified in each phase, including retromer complex components and immune mediators such as Csf3, Gsdmd, and Ifi35. Notably, bacterial proteins were consistently detected in exosomes: GroEL and SodC were present at both 8 and 24 hpi, whereas Omp19, Omp2b, DnaK, and the invasion protein B homolog BAB1_0368 were restricted to the early phase. These findings indicate that exosomes derived from B. abortus–infected macrophages are not mere byproducts, but active mediators of bacterial persistence and immune modulation, with potential as biomarkers and therapeutic tools.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-14_10:16:10.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillermo Nourdin
SpeciesList	scientific name: Brucella abortus str. 2308 A; NCBI TaxID: NEWT:641140;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-06 08:08:58	ID requested
⏵ 1	2026-05-14 10:16:10	announced

10.3389/FIMMU.2025.1685245;

submitter keyword: Macrophages,Brucella abortus 2308, Immune evasion, Bacterial persistence, Exosomes, Extracellular vesicles

Angel A. Oñate
contact affiliation	Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepcion, Concepcion-Chile.
contact email	aonate@udec.cl
lab head
Guillermo Nourdin
contact affiliation	MELISA Institute
contact email	gnourdin@melisainstitute.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066999
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Exosomes from Brucella abortus infected Macrophages Reveals Mechanisms of Immune Evasion and Host Modulation