PXD066956

PXD066956 is an original dataset announced via ProteomeXchange.

Title	TMT proteome analysis of mouse pancreatic tissue
Description	Pancreatic tissues were collected from mice that had received 2 weeks of chronic treatment. After anesthetized with isoflurane, tissues were snap-frozen in liquid nitrogen and stored at -80℃. Protein concentration was determined, and then 100 μL 0.1 M TEAB buffer and 41 μL TMT labeling reagent (dissolved in acetonitrile) were added to the samples. After a 2-hour incubation, the reaction was terminated by adding 8% ammonia water. The labeled samples were combined in equal volumes, desalted, and lyophilized. The lyophilized samples were resuspended in mobile phase A (2% acetonitrile, pH adjusted to 10.0 with ammonium hydroxide) and subjected to gradient elution using mobile phase B (98% acetonitrile). Samples were centrifuged at 14,000 g for 20 min at 4℃. For analysis, an L-3000 HPLC system equipped with a Water BEH C18 column (4.6 × 250 mm, 5 μm) was used, with the column maintained at 45 °C. Secondary mass spectrometry, was performed using a Q ExactiveTM series mass spectrometer. The resolution was set to 45000 (at 200 m/z), the maximum capacity of C-trap was 5 × 104, and the maximum C-trap injection time was 86 ms. Peptide fragmentation was achieved using a 32% collision energy, with a threshold intensity of 1.2 × 105.
HostingRepository	iProX
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-04_20:15:25.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Hong
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-04 20:15:05	ID requested
⏵ 1	2025-08-04 20:15:26	announced

Dataset with its publication pending

submitter keyword: TMT proteome, mouse, pancrease

Zuxin Chen
contact affiliation	Shenzhen Institute of Advanced Technology
contact email	zx.chen3@siat.ac.cn
lab head
Feng Hong
contact affiliation	Shenzhen Institute of Advanced Technology
contact email	hongfengshengli@163.com
dataset submitter

iProX dataset URI