PXD066894

PXD066894 is an original dataset announced via ProteomeXchange.

Title	Coordination of cyanobacterial nitrate assimilation and photosynthesis by a novel PsbO-interacting protein PirN
Description	Nitrogen assimilation relies on photosynthetically-produced energy and reducing equivalents. Here, we identify a small protein, PsbO-interacting regulator of nitrate assimilation (PirN), as a key coordinator of photosynthesis and nitrate assimilation in Synechocystis sp. PCC 6803 (Synechocystis). PirN specifically accumulates under nitrate conditions and interacts with the photosystem II (PSII) subunit PsbO. Deletion of pirN (ΔpirN) under nitrate impairs growth, reduces PSII contents and oxygen evolution, and upregulates most nitrogen transport and assimilation proteins except the nitrate reductase NarB, mimicking nitrogen starvation response likely due to impaired nitrate reduction. PsbO expression is markedly reduced in both ΔpirN and ΔnarB. Complementation of NarB or PsbO in ΔpirN largely restores mutant growth and the expression of the other, although to different extents. These findings suggest that PirN is a nitrate-inducible regulator that hierarchically modulates NarB and PsbO expression to couple nitrate assimilation with photosynthetic activity. This coordination likely safeguards redox homeostasis when nitrate assimilation or photosynthetic electron production is perturbed.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:52:52.613.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengcheng Huang
SpeciesList	scientific name: Synechocystis sp. PCC 6803 substr. GT-T; NCBI TaxID: NEWT:2930568;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-03 13:26:12	ID requested
⏵ 1	2026-06-07 16:52:53	announced

10.1002/advs.202518047;

Huang C, Xiao Z, Ge H, Wang G, Dong J, Wang Y, Yang H, Wang X, Gao H, Wang Z, Yang H, Zhang Y, Huang X, Xu W, Ma W, Yang W, Wang Y, Coordination of Cyanobacterial Nitrate Assimilation and Photosynthesis by a Novel PsbO-Interacting Protein PirN. Adv Sci (Weinh), 13(29):e18047(2026) [pubmed]

submitter keyword: cyanobacteria,photosynthesis, PsbO, nitrate assimilation, PirN

Wang Yingchun
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Rd., Beijing 100101, China.
contact email	ycwang@genetics.ac.cn
lab head
Chengcheng Huang
contact affiliation	University of Chinese Academy of Sciences
contact email	cc.huang@genetics.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD066894

PRIDE project URI

• PRIDE
  1. PXD066894
     1. Label: PRIDE project
     2. Name: Coordination of cyanobacterial nitrate assimilation and photosynthesis by a novel PsbO-interacting protein PirN