PXD066892

PXD066892 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana_Col-0_ces-qM_0and7dpi_Hpa
Description	When organisms encounter pathogens, they rapidly activate complex defense programs to ensure survival. While these immune responses are vital, they often also incur trade-offs, such as reduced growth and development and must therefore be tightly controlled. In this study, we reveal that the steroid hormones brassinosteroids (BRs) contribute to this control in Arabidopsis thaliana by repressing immunity-related genes. We provide evidence that the BR-regulated basic helix-loop-helix (bHLH) transcription factor CESTA (CES), along with its homologs BR ENHANCED EXPRESSION (BEE)1-3, mediate DNA methylation changes at transposable element (TE)-rich loci containing nucleotide-binding leucine-rich-repeat (NLR)-type receptor genes, including SUPPRESSOR OF NPR1-1 CONSTITUTIVE 1 (SNC1). These CES-induced methylation changes correlate with altered splicing of SNC1 pre-mRNA, a process that requires the BR receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1). In support, we show that CES associates with components of the chromatin remodeling and splicing machinery. Together, our findings reveal a previously unrecognized BR-induced mechanism that modulates the epigenetic and post transcriptional regulation of immune genes, enabling plants to prioritize growth over defense.
HostingRepository	PRIDE
AnnounceDate	2026-08-03
AnnouncementXML	Submission_2026-08-03_01:09:23.166.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD066892
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Veronica Ramirez
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-03 11:04:25	ID requested
⏵ 1	2026-08-03 01:09:23	announced

10.6019/PXD066892;

10.1073/PNAS.2532739123;

submitter keyword: Arabidopsis thaliana, CESTA, Hpa

Prof. Dr. Brigitte Poppenberger
contact affiliation	Biotechnology of Horticultural Crops, Technical University of Munich
contact email	brigitte.poppenberger@tum.de
lab head
Veronica Ramirez
contact affiliation	Technical University of Munich
contact email	veronicae.ramirez@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD066892

PRIDE project URI

• PRIDE
  1. PXD066892
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana_Col-0_ces-qM_0and7dpi_Hpa