⮝ Full datasets listing
PXD066854
PXD066854 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | VCPIP1 Drives Diabetic Cardiomyopathy by Deubiquitinating AMPKγ1 and Preventing AMPKα-γ Subunit Assembly in Cardiomyocytes |
| Description | Cardiomyocytes were transfected with either Flag-VCPIP1 or Flag-empty vector (EV) plasmid for ubiquitin proteomics analysis. In brief, cell samples were lysed ,and the supernatant was collected and protein concentration was determined. For each sample, the protein was reduced with dithiothreitol at 56°C and subsequently alkylated with iodoacetamide at room temperature in the dark. The proteins were first digested with trypsin at a trypsin-to-protein ratio overnight at 37°C, followed by a second digestion at 37°C. The resulting peptides were divided for ubiquitin peptide enrichment and protein quantification. For ubiquitin remnant enrichment, peptides were dissolved in NETN buffer and incubated with pre-washed antibody beads at 4°C overnight with gentle agitation. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-03-16 |
| AnnouncementXML | Submission_2026-03-16_00:51:26.757.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | guoxuan liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | ubiquitinylated lysine |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-01 02:43:09 | ID requested | |
| ⏵ 1 | 2026-03-16 00:51:27 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: VCPIP1；AMPKγ1 |
Contact List
| XUE HAN | |
|---|---|
| lab head | |
| guoxuan liu | |
| contact affiliation | Hangzhou Medical College |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003971/ |
