PXD066844

PXD066844 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mannosidic glycans on misfolded Arabidopsis SUBEX glycopeptides
Description	The mutated extracellular domain of Arabidopsis STRUBBELIG (SUBEX) was fused to GFP and either transiently expressed in Nicotiana benthamiana leaves or stably in Arabidopsis thaliana seedlings and purified. The purified SUBEX proteins were subjected to SDS-PAGE and CBB staining. Corresponding gel pieces were excised and the proteins digested with proteases and jack bean alpha-mannosidase. Glycopeptides were analysed by MS to identify the N-glycan structures. In addition, glycans were released from SUBEX peptides using PNGase A and analysed by PGC-LC-MS.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_16:14:06.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Strasser
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; scientific name: Nicotiana benthamiana; NCBI TaxID: NEWT:4100;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 23:49:40	ID requested
⏵ 1	2025-10-12 16:14:07	announced

Publication List

10.1016/j.jbc.2025.110649;
Shin YJ, Vavra U, Maresch D, Gr, ü, nwald-Gruber C, Strasser R, Mannose trimming is the dominant signal for the release of misfolded glycoproteins from ER quality control. J Biol Chem, 301(10):110649(2025) [pubmed]

10.1016/j.jbc.2025.110649;

Shin YJ, Vavra U, Maresch D, Gr, ü, nwald-Gruber C, Strasser R, Mannose trimming is the dominant signal for the release of misfolded glycoproteins from ER quality control. J Biol Chem, 301(10):110649(2025) [pubmed]

Keyword List

submitter keyword: endoplasmic reticulum‐associated protein degradation (ERAD), endoplasmic reticulum (ER),Cell biology, glycoprotein, N-linked glycosylation, protein degradation, plant

submitter keyword: endoplasmic reticulum‐associated protein degradation (ERAD), endoplasmic reticulum (ER),Cell biology, glycoprotein, N-linked glycosylation, protein degradation, plant

Contact List

Richard Strasser
contact affiliation	BOKU University, Vienna, Austria
contact email	richard.strasser@boku.ac.at
lab head
Richard Strasser
contact affiliation	University of Natural Resources and Life Sciences Vienna
contact email	richard.strasser@boku.ac.at
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066844
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066844

PRIDE project URI

Repository Record List

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