PXD066843

PXD066843 is an original dataset announced via ProteomeXchange.

Title	Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia
Description	This dataset contains mass spectrometry raw files and identification results from intact N-glycoproteomic analysis of extracellular vesicles (EVs) isolated from human seminal plasma. Intact N-glycopeptides were enriched and analyzed using an Orbitrap Fusion Lumos mass spectrometer coupled to an Easy-nLC 1200 system. The uploaded dataset includes: (1) Data-dependent acquisition (DDA) LC-MS/MS files acquired via stepped collision energy higher-energy collision dissociation (StepHCD) across discovery cohort samples; and (2) StepHCD-based parallel reaction monitoring (PRM) targeted LC-MS/MS files across validation cohort samples. Raw data files were searched against the human UniProt reviewed database using Glyco-Decipher software with a 1% false discovery rate (FDR).
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-24_02:18:28.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingyang Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 22:49:04	ID requested
1	2026-08-14 20:16:09	announced
⏵ 2	2026-08-24 02:18:28	announced	2026-08-24: Updated project metadata.

Dataset with its publication pending

submitter keyword: intact glycoproteomic,Azoospermia, seminal plasma, extracellular vesicles

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China
contact email	guo_xuejiang@njmu.edu.cn
lab head
Mingyang Li
contact affiliation	Southeast University
contact email	230229239@seu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD066843

PRIDE project URI

• PRIDE
  1. PXD066843
     1. Label: PRIDE project
     2. Name: Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia