PXD066833

PXD066833 is an original dataset announced via ProteomeXchange.

Title	Uncovering a Fibroblast Differentiation-Based Keloid Classification by Integration of Single-cell and Bulk RNA Sequencing
Description	Keloids are dermal fibroproliferative skin disorders caused by abnormal wound healing, resulting in impaired skin function and aesthetic defects. Abnormal fibroblast proliferation and excessive collagen deposition are involved in keloid formation. This study investigated the role of fibroblast differentiation in keloid development. Single-cell and bulk RNA sequencing data of keloids were comprehensively analyzed, and 25 clinically relevant differentially expressed fibroblast-differentiation-related genes (DEFDRGs) were identified. Based on DEFDRGs, a keloid diagnostic classification system comprising three subtypes was constructed, indicating that DEFDRGs could serve as therapeutic targets. Additionally, multiple microarray datasets, protein sequencing data, and immunohistochemical analyses of key markers in clinical keloid samples were used for further verification. In conclusion, this study established a molecular classification of keloids based on fibroblast differentiation, contributing to the further understanding of keloid pathogenesis and providing new insights for diagnosis and treatment.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:44:06.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 18:49:39	ID requested
⏵ 1	2025-10-05 16:44:07	announced

10.1038/s42003-025-08741-1;

Zhang W, Lu J, Tong X, Xie S, Xian S, Liu Y, Yan J, Qian W, Sun H, Zhu Y, Jiang L, Guo X, Ding X, Li Y, Bai C, Huang R, Xia Z, Ji S, Uncovering a fibroblast differentiation-based keloid classification by integration of single-cell and bulk RNA sequencing. Commun Biol, 8(1):1387(2025) [pubmed]

submitter keyword: Keloids

Fibroblasts

Differentiation

Molecular Classification

Shizhao Ji
contact affiliation	Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Shanghai, People’s Republic of China; Research Unit of key techniques for treatment of burns and combined burns and trauma injury, Chinese Academy of Medical Sciences, Shanghai, China.
contact email	shizhaoji2022@163.com
lab head
Wei Zhang
contact affiliation	Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People’s Republic of China
contact email	15821680963@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066833

PRIDE project URI

• PRIDE
  1. PXD066833
     1. Label: PRIDE project
     2. Name: Uncovering a Fibroblast Differentiation-Based Keloid Classification by Integration of Single-cell and Bulk RNA Sequencing