PXD066810 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in mice |
| Description | Accumulating evidence suggests that a compromised intestinal epithelial barrier (IEB) contributes to the progression of metabolic dysfunction-associated steatotic liver disease (MASLD); however, the exact mechanisms remain unclear. Here we reveal that intestinal mucin 1 (MUC1) levels and glycosylation are decreased in both humans and mice with MASLD. Enterocyte-specific Muc1 knockout aggravates high-fat diet (HFD)-induced IEB impairment and MASLD progression in mice. Mechanistically, HFD feeding reduces the glycosylation of intestinal epithelial MUC1, triggering its clathrin-mediated endocytosis and NEDD4-mediated lysosomal degradation, which subsequently induces β-Catenin degradation and ultimately impaires the IEB. Notably, enterocyte-specific overexpression of cytoplasmic-tail-deleted MUC1 protects against IEB impairment and mitigates MASLD progression. These findings indicate that reduced intestinal epithelial MUC1 levels facilitate the progression of MASLD. Preserving the glycosylation and levels of intestinal MUC1 to maintain IEB integrity is a potential therapeutic strategy to explore for MASLD. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-14 |
| AnnouncementXML | Submission_2025-12-14_11:49:14.860.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zecheng Li |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-31 09:51:08 | ID requested | |
| ⏵ 1 | 2025-12-14 11:49:15 | announced | |
Publication List
| Li Z, Gu R, Liu D, Zhang Y, Qin Y, Li X, Qiao Y, Li Z, Guo J, Wang J, Meng H, Peng L, Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in male mice. Nat Commun, 17(1):321(2025) [pubmed] |
| 10.1038/s41467-025-67034-7; |
Keyword List
| submitter keyword: MASLD |
| intestinal epithelial barrier |
| MUC1 |
| glycosylation |
| β-catenin |
Contact List
| Liang Peng |
|---|
| contact affiliation | Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China |
| contact email | pengliang@zryhyy.com.cn |
| lab head | |
| Zecheng Li |
|---|
| contact affiliation | China Japan Friendship Hospital |
| contact email | pumc2024@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD066810 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066810
- Label: PRIDE project
- Name: Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in mice
