PXD066799

PXD066799 is an original dataset announced via ProteomeXchange.

Title	Filamentous growth, cell envelope architecture and surface appendages in Litorilinea aerophila
Description	Litorilinea aerophila, a filamentous member of the phylum Chloroflexota (class Caldilineae), exhibits unique morphological and cell envelope features that challenge traditional bacterial models. Although historically described as Gram-negative, Chloroflexota are increasingly considered as monoderm, lacking a true outer membrane. In this study, we investigated the growth behaviour, cell morphology, and cell appendages of Litorilinea aerophila using a combination of microscopy, electron tomography, and structural biology. Dry weight-based growth assays revealed a prolonged lag phase (~40 h) followed by exponential and stationary phases. Light and fluorescence microscopy revealed irregular constrictions along cell filaments, accompanied by a diffuse distribution of DNA, indicating a multicellular organization. Thin-section electron microscopy confirmed septa formation, and in late growth stages, filaments became shorter with more defined constrictions and membrane vesicle release. Next to the already characterised bacterial archaellum of Litorilinea aerophila, two additional types of surface appendages were identified: (i) pilus-like structures consistent with Tad pili, supported by comparative genomics, and (ii) grappling hook-like structures. These hook-like structures were related to a DUF11 domain-containing protein that was verified by quantitative mass spectrometry. These findings contribute to the understanding of cell envelope diversity, filamentous growth, and surface structures in Chloroflexota, revealing evolutionary plasticity within this enigmatic bacterial phylum.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_04:23:33.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pitter Huesgen
SpeciesList	scientific name: Litorilinea aerophila; NCBI TaxID: NEWT:1204385; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 06:46:08	ID requested
⏵ 1	2026-06-09 04:23:33	announced

Dataset with its publication pending

submitter keyword: cell surface structures, cell envelope,Chloroflexota, LC-MS/MS

Pitter Huesgen
contact affiliation	Faculty of Biology, University of Freiburg, Freiburg, Germany
contact email	pitter.huesgen@biologie.uni-freiburg.de
lab head
Pitter Huesgen
contact affiliation	University of Freiburg Faculty of Biology Biochemistry and Functional Proteomics
contact email	pitter.huesgen@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066799
     1. Label: PRIDE project
     2. Name: Filamentous growth, cell envelope architecture and surface appendages in Litorilinea aerophila