PXD066794

PXD066794 is an original dataset announced via ProteomeXchange.

Title	Paleoproteomics in fossil amber arthropods
Description	Paleoproteomics is the proteomics analysis of ancient proteins, which may be better preserved than DNA in fossil inclusions and particularly in amber fossilized tree resin. However, only yeast proteins have been previously suggested as identified by paleoproteomics analysis conducted in fossil amber. In this study, we developed and applied a paleoproteomics approach to study fossil arthropod inclusions in five Burmese (also known as Burmite and Kachin) amber (Cretaceous, ca. 99 mya) pieces. The results of morphological analysis supported the first report of fossil scavenger mite (Holothyrida, Neothyridae) together with co-inclusions of putative lacewing larva (Neuroptera, Chrysopidae) and thrip Frankliniella sp. (Thysanoptera, Thripidae), the identification of Cornupalpatum sp. (Ixodidae), and two mites Prostigmata (Acari, Trombidiformes, Tetranychidae). A control amber fragment derived from the piece with mite-lacewing-thrip co-inclusions was included in the analysis. The paleoproteomics analysis was conducted by Mass Spectrometry (MS) and Liquid Chromatography-Tandem Mass Spectrometry by searching results from all fossil amber pieces against a compile database containing all sequences from Acari, Insecta and Enterobacterales taxonomies in Uniprot together with contaminants database supplemented with human keratins and bovine trypsin. Identified proteins were selected for analysis based on those identified by MS with more than two peptides per protein and at least one peptide with 1% FDR. Paleoproteomics identified protein orthologs present in fossil amber inclusions of ca. 99 mya in arthropods and other organisms. These results provide a paleoproteomics approach to complement morphological studies in amber inclusions.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_03:39:08.411.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066794
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Margarita Villar
SpeciesList	scientific name: fossil metagenome; NCBI TaxID: NEWT:444079;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 03:37:14	ID requested
⏵ 1	2026-03-23 03:39:08	announced

10.6019/PXD066794;

submitter keyword: amber

arthropod

evolution

fossil

paleontology

paleoproteomics

Marga Villar
contact affiliation	SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain.
contact email	margaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliation	University of Castilla- La Mancha
contact email	margaritam.villar@uclm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD066794

PRIDE project URI

• PRIDE
  1. PXD066794
     1. Label: PRIDE project
     2. Name: Paleoproteomics in fossil amber arthropods