PXD066788

PXD066788 is an original dataset announced via ProteomeXchange.

Title	Golgi apparatus-associated secretome deciphering in living cells enabled by aggregation-induced emission luminogen-mediated photocatalytic proximity labeling
Description	Secretome is intimately linked to Golgi apparatus (GA) which acts as a transition platform of proteins processing and sorting in trafficking pathways. GA-associated secretome runs through the endomembrane system and is critical for inter- and intracellular communication networks. However, achieving in situ dissection of GA-associated secretome remains challenging owing to the lack of efficient labeling methods. Herein, we described an aggregation-induced emission luminogen-mediated photocatalytic proximity labeling strategy (AIE-PhoPL) that allowed dynamic profiling of GA-associated secretome with high spatiotemporal precision in minutes. With the GA-targeting photocatalyst based on aggregation-induced emission luminogen (AIEgen), the proteins were labeled locally in Golgi lumen with high efficiency under light, enabling spatiotemporally resolved modifying of histidine and tyrosine residues. AIE-PhoPL strategy was successfully utilized in living HeLa and hard-to-transfect macrophage HMC3 cells by determining a significant subset of GA-associated secretome with 80% specificity, linking the distinct GA-associated secretory profiles to cellular characteristics. We further applied AIE-PhoPL to dynamic proteome mapping of brain and bone metastatic lung cancer cells, which revealed GA-associated secretory proteomic patterns related to metastatic mechanisms and the underlying roles GA-associated secretion played in extracellular matrix organization during metastasis. This work provides a powerful tool to break out the dilemma for chemical labeling of GA-associated secretome in living cells and fuels the research on diversification of controllable chemical probes for subcellular proteome labeling.
HostingRepository	iProX
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_20:22:15.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 20:20:54	ID requested
⏵ 1	2025-07-30 20:22:16	announced

Dataset with its publication pending

submitter keyword: aggregation-induced emission, Golgi apparatus, secretome, photocatalytic proximity labelling, labeling mechanism

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
He Wang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	crane_wanghe@dicp.ac.cn
dataset submitter

iProX dataset URI