PXD066777

PXD066777 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium tuberculosis Sec and Tat secretion pathways play a role in the maintenance of membrane integrity
Description	Mycobacterium tuberculosis (Mtb) secretes many proteins into the host to modulate various cellular processes, such as host signaling, immune responses, apoptosis, etc., for survival. Mtb protein secretion comprises three different secretion systems: Sec dependent, Sec independent (Tat), and Type VII (T7SS) secretion pathways. CRISPR-based knockdown strains of secA1 and tatA were generated to characterize SecA1 and TatA-mediated secretion systems in Mtb. The absence of both compromised pathogen survival in vitro and ex vivo. The secretome analysis of depletion strains showed a significant decrease in pathway specific secretory proteins compared to Rv. Membrane proteomics suggested substantial changes in the membrane proteome upon depletion. SEM analysis of depleted strains changes in the cellular morphology with cells bulging from the poles upon SecA1 depletion and increasing the length upon TatA depletion. TEM analysis indicates thinning of the cell membrane and cell wall and confirms the changes in the cellular morphology in depletion strains. Together, data suggests that disruption of sec and tat pathway leads to changes in the membrane permeability.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_03:39:08.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinay Kumar Nandicoori
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: NEWT:83332; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 11:17:07	ID requested
⏵ 1	2026-03-23 03:39:09	announced

10.1016/j.mcpro.2026.101555;

Sanyal P, Uppada J, Sinha S, Bhat Y, Khan S, Rajaram SV, Arokiyaraj EA, Jhingan GD, Agarwal N, Samal A, Nandicoori VK, Sec and Tat mediated secretion safeguards Mycobacterium tuberculosis membrane homeostasis. Mol Cell Proteomics, ():101555(2026) [pubmed]

submitter keyword: None

Dr Vinay
contact affiliation	CSIR-CCMB
contact email	vinaykn.ccmb@csir.res.in
lab head
Vinay Kumar Nandicoori
contact affiliation	CSIR-CCMB
contact email	vinaykn.ccmb@csir.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD066777

PRIDE project URI

• PRIDE
  1. PXD066777
     1. Label: PRIDE project
     2. Name: Mycobacterium tuberculosis Sec and Tat secretion pathways play a role in the maintenance of membrane integrity