PXD066766 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of BRCA2-DSS1 new partner HELZ |
| Description | R-loops are dynamic three-stranded nucleic acid structures that regulate genome function but can threaten genome stability if not properly resolved. BRCA2, a key player in homologous recombination (HR), also regulates R-loops, though the mechanisms remain unclear. To uncover novel R-loop regulators, we employed a BioID-DSS1 proximity labeling system, leveraging DSS1’s stable interaction with BRCA2 to map its protein interaction network under DNA damage conditions. This approach identified HELZ, a putative RNA helicase, as a new BRCA2-DSS1 interactor. We found that HELZ functions as an RNA-specific R-loop resolvase, revealing a previously unrecognized BRCA2-HELZ axis in R-loop resolution. This work provides new insights into how BRCA2 safeguards genome integrity beyond its canonical role in HR. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-30 |
| AnnouncementXML | Submission_2025-12-30_13:52:28.373.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD066766 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Wenjing Li |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | biotinylated residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-30 07:25:43 | ID requested | |
| ⏵ 1 | 2025-12-30 13:52:28 | announced | |
Publication List
Keyword List
| submitter keyword: HeLa, BiotinID-DSS1, BRCA2, HELZ |
Contact List
| Weixing Zhao |
|---|
| contact affiliation | Biochemistry and Structural Biology Greehey Children's Cancer Research Institute University of Texas Health at San Antonio United States |
| contact email | zhaow2@uthscsa.edu |
| lab head | |
| Wenjing Li |
|---|
| contact affiliation | UT Health San Antonio |
| contact email | liw1@uthscsa.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD066766 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066766
- Label: PRIDE project
- Name: Identification of BRCA2-DSS1 new partner HELZ
