PXD066750

PXD066750 is an original dataset announced via ProteomeXchange.

Title	Global identification of ER stress-regulated RNA binding Proteins in clonal 2 pancreatic beta-cells
Description	Endoplasmic reticulum (ER) stress plays an important role in pancreatic β-cell 20 dysfunction and death, contributing to both type 1 and type 2 diabetes. The Unfolded 21 Protein Response (UPR) is a key adaptive mechanism that helps cells cope with ER 22 stress by altering gene expression. RNA-binding proteins (RBPs), which regulate gene 23 expression at multiple levels, are likely to influence the UPR, yet their role in the UPR 24 remain largely unexplored. 25 To investigate this, we examined global changes in RBP-RNA interactions in the 26 mouse β-cell line MIN6 under ER stress. This analysis identified 339 RBPs, with 13 27 showing significant changes in RNA binding. Among these, DEAD-box helicase 3 X28 linked (DDX3X) emerged as a candidate for further study. Functional experiments 29 revealed that inhibiting or knocking down DDX3X in MIN6 cells suppressed the ER 30 stress-induced expression of the pro-apoptotic protein CHOP and its upstream 31 regulator ATF4.
HostingRepository	PRIDE
AnnounceDate	2026-05-29
AnnouncementXML	Submission_2026-05-29_04:18:37.053.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 02:11:56	ID requested
⏵ 1	2026-05-29 04:18:37	announced

Dataset with its publication pending

submitter keyword: SWATH, DDX3X, Diabetes,RNA Binding Protein, RBP

David Boocock
contact affiliation	John van Geest Cancer Research Centre, Nottingham Trent University, UK
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD066750

PRIDE project URI

• PRIDE
  1. PXD066750
     1. Label: PRIDE project
     2. Name: Global identification of ER stress-regulated RNA binding Proteins in clonal 2 pancreatic beta-cells