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PXD066739

PXD066739 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleS. elongatus PCC 7942 Limited Proteolysis Structural Proteomics (JM-PB-DP3), TPP-MS-TMT10
DescriptionThe purpose of this experiment was to investigate structural alterations in proteins involved in central carbon metabolism and photosynthetic electron transfer pathways in Synechococcus elongatus PCC 7942. Sample data was obtained from S. elongatus PCC 7942 cell lysates treated across 10 temperatures (37 to 82 deg C in 5 deg C increments, prepared using thermal proteome profiling by mass spectrometry (TPP-MS), and fractionated for TMT10 proteomic analysis. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline, followed by Tandem Mass Tag 10-Plex (TMT10) quantitation using PlexedPiper.
HostingRepositoryMassIVE
AnnounceDate2025-10-16
AnnouncementXMLSubmission_2025-10-16_15:42:34.868.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Synechococcus elongatus PCC 7942; NCBI TaxID: 1140;
ModificationListOxidation; TMT6plex
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-07-29 20:02:34ID requested
12025-10-16 15:42:35announced
Publication List
no publication
Keyword List
submitter keyword: Cyanobacteria, Light Fluctuation, Structural Proteomics, Redox Proteomics, Thermal Proteome Profiling, Thiol Oxidation, DatasetType:Proteomics
Contact List
Snigdha Sarkar
contact affiliationPacific Northwest National Laboratory
contact emailsnigdha.sarkar@pnnl.gov
lab head
John T. Melchior
contact affiliationPacific Northwest National Laboratory
contact emailjohn.melchior@pnnl.gov
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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