PXD066729

PXD066729 is an original dataset announced via ProteomeXchange.

Title	Identification of bacterial secretins
Description	Cyanobacteria and myxobacteria exhibit gliding motility that is associated with the secretion of an exopolymeric slime through nozzle-like structures. Using a combination of biochemical and structural approaches, we demonstrate that these nozzles are composed of secretins belonging to the PilQ/GspD family; proteins previously characterized as forming outer membrane gates in type-two secretion systems (T2SSs) and other bacterial secretion machineries. We show that gspD is an essential gene in Myxococcus xanthus, and that its conditional knockdown impairs both A-motility-associated exopolymer (AEP) slime secretion and gliding motility. In cyanobacteria, available data indicate that the exopolymeric slime is a polysaccharide, whereas the precise composition of the slime in myxobacteria remains unresolved. These findings suggest that secretins can mediate the secretion of non-proteinaceous polymers in certain bacteria.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_10:31:47.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Zuckerman
SpeciesList	scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: NEWT:246197; scientific name: Arthrospira platensis NIES-39; NCBI TaxID: NEWT:696747; scientific name: Kamptonema sp. PCC 6506; NCBI TaxID: NEWT:272129;
ModificationList	methionine oxidation with neutral loss of 80 Da; S-carboxamidomethyl-L-cysteine; methionine oxidation with neutral loss of 64 Da; mono N-acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-29 11:12:26	ID requested
⏵ 1	2025-10-07 10:31:48	announced

Zuckerman DM, So JMT, Hoiczyk E, Secretins of type-two secretion systems are necessary for exopolymeric slime secretion in cyanobacteria and myxobacteria. Nat Commun, 16(1):8482(2025) [pubmed]

10.1038/s41467-025-63426-x;

submitter keyword: secretion, secretin,Cyanobacteria, myxobacteria, carbohydrate

Egbert Hoiczyk
contact affiliation	School of Biosciences, University of Sheffield, Sheffield, UK
contact email	egbert.hoiczyk@gmail.com
lab head
David Zuckerman
contact affiliation	Iona University
contact email	dzuckerman@iona.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066729

PRIDE project URI

• PRIDE
  1. PXD066729
     1. Label: PRIDE project
     2. Name: Identification of bacterial secretins