PXD066694

PXD066694 is an original dataset announced via ProteomeXchange.

Title	NSP15 facilitating viral immune evasion by regulating the liquid-liquid phase separation of IKKε
Description	Coronavirus evades antiviral innate immune responses through encoding multiple viral proteins, thereby facilitating viral replication. However, little is known whether different coronaviruses employ conserved proteins to universally regulate innate immunity. In this study, we found that the conserved endoribonuclease NSP15 significantly suppressed type Ι interferon response, and promotes coronavirus replication. Mechanistically, NSP15 proteins from different coronaviruses interacted with IκB kinase epsilon (IKKε), a key regulator innate immunity, and wrecked the formation of liquid-liquid phase-separated (LLPS) IKKε bodies, resulting in the degradation of IKKε and a highly impaired interferon response.
HostingRepository	iProX
AnnounceDate	2025-08-01
AnnouncementXML	Submission_2026-08-13_20:06:02.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taizhen Liang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 20:56:40	ID requested
⏵ 1	2026-08-13 20:06:02	announced

Dataset with its publication pending

submitter keyword: NSP15, IKKε, LLPS, interferon response, immune evasion

Xiancai Ma
contact affiliation	Guangzhou National Laboratory
contact email	ma_xiancai@gzlab.ac.cn
lab head
Taizhen Liang
contact affiliation	Guangzhou National Laboratory
contact email	liang_taizhen@gzlab.ac.cn
dataset submitter

iProX dataset URI