PXD066687

PXD066687 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanisms underlying the antitumoral effects of Angiotensin-(1-7) in the A549 cell line
Description	Angiotensin-(1-7) [Ang-(1-7)] is a heptapeptide of the renin-angiotensin system (RAS) with antitumoral effects reported in various tumoral cell lines, including the lung adenocarcinoma A549. While previous studies have shown that Ang-(1-7) modulates MAPK and PI3K-AKT signaling, the precise molecular mechanisms involved remain incompletely understood. To investigate the signaling events underlying the antitumoral effects of Ang-(1-7) in lung cancer-derived cells, we employed an integrated proteomic and phosphoproteomic approach in A549 cells. We analyzed early (minutes) and late (hours) molecular responses to Ang-(1-7) treatment. The treatment resulted in time-dependent modulation of multiple signaling pathways, including significant alterations in the MAPK, PI3K-AKT, and mTOR pathways at both the protein and phosphorylation levels. Notably, widespread early dephosphorylation events were observed similar to the effects seen with other RAS peptides with antitumoral effects. Additionally, Ang-(1-7) promoted nuclear accumulation of the transcription factor FOXO1 indicating its activation. FOXO1 is known to regulate genes involved in apoptosis, cell cycle arrest, and oxidative stress, suggesting a role in mediating the peptide’s antitumoral effects. The study provides new insights into the molecular basis of Ang-(1-7)’s antitumoral activity in A549 cells and reinforce its therapeutic potential in lung cancer.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-25_17:47:25.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 17:23:53	ID requested
⏵ 1	2026-01-25 17:47:28	announced

Melo-Braga MN, Magalh, ã, es GC, Silva FA, Kjeldsen F, Larsen MR, Santos RAS, Verano-Braga T, Time-resolved proteomic and phosphoproteomic profiling of Angiotensin-(1-7) signaling in A549 cells. J Proteomics, 326():105607(2026) [pubmed]

10.1016/j.jprot.2026.105607;

submitter keyword: A549 cell line, renin-angiotensin system, angiotensin-(1-7)

Thiago Verano-Braga
contact affiliation	Universidade Federal de Minas Gerais
contact email	npf.ufmg@gmail.com
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066687
     1. Label: PRIDE project
     2. Name: Molecular mechanisms underlying the antitumoral effects of Angiotensin-(1-7) in the A549 cell line