PXD066686

PXD066686 is an original dataset announced via ProteomeXchange.

Title	Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer-Cell lines
Description	High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry technologies face limitations in speed, sensitivity, and scalability for analyzing large sample cohorts. The Thermo Scientific™ Orbitrap™ Astral™ Zoom mass spectrometer (MS) was developed to address these limitations by improving acquisition speed, ion utilization, and spectral processing, which are all essential for advancing proteome depth in high-throughput proteomics. The Orbitrap Astral Zoom MS achieves ultra-fast MS/MS scan rates of up to 270 Hz with enhanced ion utilization through pre-accumulation, enabling the identification of ~100,000 unique peptides and >8,400 proteins in a single 300 samples-per-day (SPD) analysis of human cell lysate. The optimized system reduces analysis time by 40%, achieves near-complete proteome coverage (>12,000 proteins) in 2.7 hours, and enables ultra-high-throughput workflows, identifying >7,000 proteins in a 500 SPD method with exceptional reproducibility (Pairwise Pearson correlations >0.99). These advancements establish the Orbitrap Astral Zoom MS as a new benchmark in proteomics, significantly enhancing speed, sensitivity, and scalability, paving the way for routine large-scale proteome studies with applications in clinical research and systems biology.
HostingRepository	PRIDE
AnnounceDate	2026-02-07
AnnouncementXML	Submission_2026-02-06_23:43:47.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulises H Guzman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral Zoom

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 17:02:04	ID requested
⏵ 1	2026-02-06 23:43:48	announced

Guzman UH, Rykar M, Hendriks IA, Stewart H, Denisov E, Hagedorn B, Petzoldt J, Kreutzmann A, Mueller Y, Arrey TN, Colonius I, Ø, stergaard O, Koenig C, Kraegenbring J, Fort KL, Couzijn E, Hauschild JP, Hermanson D, Zabrouskov V, Hock C, Damoc E, Olsen JV, Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer. Mol Cell Proteomics, ():101504(2026) [pubmed]

10.1016/j.mcpro.2025.101504;

submitter keyword: Orbitrap Astral Zoom,HpH Fractionation

JEsper V Olsen
contact affiliation	Administrerende Direktør • Novo Nordisk Foundation Center for Protein Research
contact email	jesper.olsen@cpr.ku.dk
lab head
Ulises H Guzman
contact affiliation	Novo Nordisk Foundation Center for Protein Research
contact email	ulysseshrdz@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD066686

PRIDE project URI

• PRIDE
  1. PXD066686
     1. Label: PRIDE project
     2. Name: Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer-Cell lines