PXD066655

PXD066655 is an original dataset announced via ProteomeXchange.

Title	HeLa cell protein interactome of polyamine affinity-based probes (probe-modified peptides)
Description	HeLa cells were incubated with affinity-based probes designed as synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine. Probe-protein interactions were captured in situ using UV-induced crosslinking. After cell lysis, the probe–protein conjugates were subjected to bioorthogonal ligation with a biotinylation reagent, followed by tryptic digestion and affinity purification of the probe-labeled peptides, which were then analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-22_04:53:45.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 05:22:01	ID requested
⏵ 1	2025-09-22 04:53:45	announced

10.1039/D5CB00103J;

submitter keyword: Photoaffinity probes, polyamines, probe-modified peptides

Remigiusz Serwa
contact affiliation	IMol Institute, Polish Academy of Sciences, Warsaw, Poland
contact email	r.serwa@imol.institute
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066655

PRIDE project URI

• PRIDE
  1. PXD066655
     1. Label: PRIDE project
     2. Name: HeLa cell protein interactome of polyamine affinity-based probes (probe-modified peptides)