PXD066651

PXD066651 is an original dataset announced via ProteomeXchange.

Title	Comparative Analysis of the Fecal Proteome in Two Canine Breeds: Dalmatians and Weimaraners
Description	The analysis of proteins in stool samples can significantly enhance the study of mamma-lian physiology and disease. In this study, we investigated the fecal proteome of clinically healthy dogs (n = 26) by a label-free proteomics approach to evaluate the impact of breed differences. The dogs were split into two groups (n = 13 each) based on their breed, specif-ically Weimaraner and Dalmatian, the former known for their possible susceptibility to gastrointestinal disease. Quantitative and qualitative differences between the two experi-mental groups were identified based on analyses performed on pooled biological sam-ples,. The overall fecal proteome profile comprised 58 proteins, of which 37 were in com-mon, while the comparative proteomics analysis detected 15 proteins having different abundance. Notably, the fecal proteome of Weimaraners showed an over-representation of proteins such as pantetheinase, which promotes inflammatory reactions; ferritin heavy chain and hemoglobin, possibly associated with gut ulceration and/or rectal bleeding typical of IBD; and anionic trypsin, implicated in inflammatory bowel disease. Notably, in Dalmatians, despite the absence of specific predispositions, some proteins associated with chronic enteropathy (e.g., carboxypeptidase B and serine protease 1) were also over-represented. Additionally, some proteins linked to breed variation included enzymes associated with “protein digestion and absorption” and “glycolysis and gluconeogene-sis”. These findings suggest, for the first time, that the variable breed is a factor potentially able to influence the fecal proteome in dogs.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-21_16:18:21.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	daniela sorio
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 01:48:03	ID requested
⏵ 1	2025-09-21 16:18:22	announced

10.3390/ijms26178247;

Cerquetella M, Pinnella F, Morazzini R, Rossi G, Marchegiani A, Gavazza A, Mangiaterra S, Di Cerbo A, Sorio D, Brandi J, Cecconi D, Vincenzetti S, Comparative Analysis of the Fecal Proteome in Two Canine Breeds: Dalmatians and Weimaraners. Int J Mol Sci, 26(17):(2025) [pubmed]

submitter keyword: dog

fecal proteome

biomarker

breed influence

pathogenesis.

Cerquetella Matteo
contact affiliation	School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, MC, Italy.
contact email	daniela.sorio@univr.it
lab head
daniela sorio
contact affiliation	Centro Piattaforme Tecnologieche Universityof Verona
contact email	daniela.sorio@univr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066651

PRIDE project URI

• PRIDE
  1. PXD066651
     1. Label: PRIDE project
     2. Name: Comparative Analysis of the Fecal Proteome in Two Canine Breeds: Dalmatians and Weimaraners