PXD066646

PXD066646 is an original dataset announced via ProteomeXchange.

Title	Venomics across the Bothrops neuwiedi species complex reveals a P-III SVMP/K49-PLA2 dichotomy and a remarkable paraspecific neutralization of the Brazilian pentabothropic antivenom
Description	Snakes of the Bothrops neuwiedi complex have a widespread distribution, and represent medically important species in Brazil. Here, we report a compositional and functional profiles of the venom of seven species of the B. neuwiedi group: B. mattogrossensis, B. pauloensis, B. pubescens, B. diporus, B. neuwiedi, B. marmoratus and B. erythromelas. The toxin composition of individual and pooled venoms showed remarkable inter- and intraspecific variability of the relative abundance of toxins (evidenced by SDS-PAGE and RP-HPLC) and enzymatic activities (proteolytic, PLA2 and thrombin-like activities). In vivo analyses showed that B. erythromelas is the most haemorrhagic venom, B. diporus was the most lethal and B. pubescens venom showed the highest myotoxic activity. Histopathological analysis showed that the venom of the seven species caused edema, haemorrhage, inflammatory infiltrate and necrosis of muscle fibers. In line with large research evidence on the paraspecificity of various commercial antivenoms generated in Latin America for the treatment of human bothropic envenomings, the pentabothropic antivenom produced by Instituto Butantan showed a high profile of immunoreactivity and lethality neutralization capability towards the venoms of the seven species of the B. neuwiedi clade. Interpreted through the prism of Evolution, our data revealed a PIII-SVMP/K49-PLA2 compositional dichotomy and a remarkable conservation of immunological crossreactivity across congeneric venoms throughout the 12-16 million years of Bothrops phylogeny.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:29:30.884.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordi Tena-Garces
SpeciesList	scientific name: Bothrops mattogrossensis; NCBI TaxID: NEWT:1171125;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt G2 HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 00:13:59	ID requested
⏵ 1	2026-02-22 16:29:31	announced

10.1021/acs.jproteome.5c00933;

Galizio NDC, Serino-Silva C, Fabri Bittencourt Rodrigues C, Stuginski DR, Rocha MMTD, Serapicos EO, Barbarini CC, Oliveira RB, Sant'Anna SS, Grego KF, Sanz L, Tena-Garc, é, s J, de Roodt AR, Calvete JJ, Tanaka-Azevedo AM, de Morais-Zani K, Dichotomy and a Remarkable Paraspecific Neutralization of the Brazilian Pentabothropic Antivenom. J Proteome Res, 25(2):1095-1114(2026) [pubmed]

ProteomeXchange project tag: Toxicoproteomics (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Bothrops venom variability

venom phenotypic dichotomy

antivenomics

immunological crossreactivity

B. neuwiedi clade

Juan José Calvete Chornet
contact affiliation	Evolutionary and translational venomics Lab, Instituto de Biomedicina de Valencia, CSIC
contact email	jcalvete@ibv.csic.es
lab head
Jordi Tena-Garces
contact affiliation	Instituto de Biomedicina de Valencia - CSIC
contact email	jtena@ibv.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD066646

PRIDE project URI

• PRIDE
  1. PXD066646
     1. Label: PRIDE project
     2. Name: Venomics across the Bothrops neuwiedi species complex reveals a P-III SVMP/K49-PLA2 dichotomy and a remarkable paraspecific neutralization of the Brazilian pentabothropic antivenom