PXD066626

PXD066626 is an original dataset announced via ProteomeXchange.

Title	Proteome, acetylome, and succinylome analysis of control and SDH-loss imCCs and iMEFs as models of SDH-loss pheochromocytoma and paraganglioma
Description	The tricarboxylic acid (TCA) cycle and electron transport chain (ETC) are key metabolic pathways required for cellular energy production. While loss of components in these pathways typically impairs cell survival, such defects can paradoxically promote tumorigenesis in certain cell types. One such example is loss of succinate dehydrogenase (SDH), which functions in both the TCA cycle and as Complex II of the ETC. Deleterious mutations in SDH subunits can cause pheochromocytoma and paraganglioma (PPGL), rare hereditary neuroendocrine tumors of chromaffin cells in the adrenal gland and the nerve ganglia, respectively. Why tumor formation upon SDH loss is limited to certain tissues remains unclear. We hypothesized that the metabolic and proteomic perturbations resulting from SDH loss are cell-type specific, favoring survival of chromaffin cells. To test this, we examined effects of SDH loss in two cell models, immortalized mouse chromaffin cells (imCCs) and immortalized mouse embryonic fibroblasts (iMEFs). We report that SDH loss differentially impacts the proteomes and acylproteomes of imCCs and iMEFs, with compartment-specific effects. Notably, SDH-loss imCCs show significant upregulation of mitochondrial proteins, including TCA cycle and fatty acid β-oxidation (FAO) enzymes, with pronounced downregulation of nuclear proteins. Both imCCs and iMEFs experience significant energy deficiency upon SDH loss, but FAO activity is uniquely increased in SDH-loss imCCs. While SDH loss increases both lysine-reactive acetyl-CoA and succinyl-CoA, SDH-loss imCCs and iMEFs show disproportionate hyperacetylation but mixed succinylation. Surprisingly, SDH-loss imCCs, but not iMEFs, display disproportionate hypoacetylation and hyposuccinylation of mitochondrial proteins. These findings suggest that cell type-specific adaptations to SDH loss underlie tissue-specific susceptibility to tumorigenesis and could illuminate therapeutic vulnerabilities of SDH-loss tumors.
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_10:28:42.940.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD066626
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sherry Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; N6-succinyl-L-lysine; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-27 19:08:11	ID requested
⏵ 1	2026-04-07 10:28:43	announced

10.1101/2025.08.01.668168;

Zhou SX, Madden B, Charlesworth MC, Hitosugi T, Favier J, Maher LJ, Distinct proteomic and acylproteomic adaptations to succinate dehydrogenase loss in two cell contexts. bioRxiv, ():(2025) [pubmed]

10.6019/PXD066626;

submitter keyword: succinylation, proteomics, acetylation, paraganglioma,pheochromocytoma, succinate dehydrogenase, acylation

L. James Maher, III
contact affiliation	Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA
contact email	maher@mayo.edu
lab head
Sherry Zhou
contact affiliation	Mayo Clinic
contact email	zhou.sherry@mayo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066626
     1. Label: PRIDE project
     2. Name: Proteome, acetylome, and succinylome analysis of control and SDH-loss imCCs and iMEFs as models of SDH-loss pheochromocytoma and paraganglioma