⮝ Full datasets listing
PXD066609
PXD066609 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Discovery of CD4+ T cell-recognized B. pertussis antigens that reduce airway colonization |
| Description | Shamseldin MM, Hall JM, Lawrence GM, Hernandez GM, Liu Y, Aubrey AR, Verzani EK, Deora R, Lovett-Racke A, Abelin JG, Dubey P. 2026. Despite widespread vaccination, Bordetella pertussis (Bp) cases are resurging globally. Although CD4+ T cells are known to be essential for sustained protection, the antigens they recognize are not fully characterized, hindering vaccine refinement. Using immunopeptidomics, bioinformatics, and functional T cell assays, we identified high-affinity epitopes from reference and clinical Bp strains presented on MHC-II I-Ab. A subset of these epitopes stimulated systemic and mucosal CD4+ T cells of mice immunized with heat-killed Bp, and peripheral blood T cells from humans vaccinated with the whole-cell pertussis vaccine. Mice immunized with a subunit vaccine comprising two recombinant proteins identified in our screen, were subsequently challenged with Bp. Bacterial burden was nearly eliminated from the lower respiratory tract and significantly reduced in the upper respiratory tract. TH1/TH17-polarized CD4+ tissue-resident memory T cells (TRM) were induced in nasal and pulmonary tissues. Depleting memory CD4+ T cells prior to challenge abolished protection, confirming that antigen-specific CD4+ T cells are critical for clearing Bp from the respiratory tract. Our integrated antigen identification and T cell assay approach revealed previously untested Bp antigens that elicit protective CD4+ T cell mediated immunity, suggesting that incorporating them into new vaccines may help curb the resurgence of pertussis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-20 |
| AnnouncementXML | Submission_2026-07-20_10:09:13.405.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Karl Clauser |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Bordetella pertussis; NCBI TaxID: 520; |
| ModificationList | unknown modification; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-26 08:45:04 | ID requested | |
| ⏵ 1 | 2026-07-20 10:09:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Whooping cough, immunopeptidomics, DatasetType:Proteomics |
Contact List
| Jennifer G. Abelin | |
|---|---|
| contact affiliation | Broad Institute |
| contact email | jabelin@broadinstitute.org |
| lab head | |
| Karl Clauser | |
| contact affiliation | Broad Institute of MIT and Harvard |
| contact email | clauser@broadinstitute.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098648/ |
