PXD066596

PXD066596 is an original dataset announced via ProteomeXchange.

Title	HDAC11 Deacetylates BRAF to Regulate Kinase Activity and Cell Proliferation
Description	Histone acetylation, governed by histone deacetylase (HDAC) enzymes, plays a pivotal role in cell biology. Elevated HDAC expression is linked to poor prognosis in various diseases, including cancer, making HDAC inhibitors clinically valuable. Among the 11 metal-dependent HDAC isoforms, the exceptional ability of HDAC11 to regulate both deacetylation and defattyacylation of proteins suggests an expansive role in cellular processes. However, since HDAC11 is one of the least studied HDAC isoforms, the known roles for HDAC11 in cell biology are limited. In this study, proteomics-based mutant trapping was performed to identify non-histone substrates of HDAC11 and link HDAC11 activity to specific cellular events. Proteomics analysis revealed 64 putative substrates, with follow-up studies documenting that HDAC11 deacetylates the BRAF kinase on K680 to suppress kinase activity and cell proliferation. Given the established role of BRAF in cancer, the data suggest that HDAC11-mediated deacetylation influences signaling pathways in tumor progression, underscoring the diverse regulatory role for HDAC11 in cellular events.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:11:25.461.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD066596
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mary Kay Pflum
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-25 10:57:47	ID requested
⏵ 1	2026-04-26 16:11:26	announced

10.6019/PXD066596;

10.1021/acschembio.5c00631;

Madueke AC, Andrade R, Pflum MKH, HDAC11 Deacetylates BRAF to Regulate Kinase Activity and Cell Proliferation. ACS Chem Biol, 20(12):2873-2885(2025) [pubmed]

submitter keyword: proteomics, BRAF, substrate profiling, kinase activity,histone deacetylase, HDAC11

Mary Kay Pflum
contact affiliation	Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202
contact email	pflum@wayne.edu
lab head
Mary Kay Pflum
contact affiliation	Wayne State University Chemistry
contact email	pflum@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066596
     1. Label: PRIDE project
     2. Name: HDAC11 Deacetylates BRAF to Regulate Kinase Activity and Cell Proliferation