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PXD066589
PXD066589 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative Muscle Proteomics in Organic and Conventional Broiler Chickens: Uncovering Biomarkers of Stress and Meat Quality |
| Description | This study investigates the impact of stress on muscle physiology and meat quality in broiler chickens by comparing protein expression profiles between organic and conventional farming systems using label-free quantitative (LFQ) proteomics. Muscle samples were analyzed via nanoLC-ESI-MS/MS coupled with comprehensive bioinformatics to identify differences in protein abundance associated with rearing conditions.A total of 7,221 proteins were identified, with 1,645 proteins upregulated and 1,612 downregulated in organic chickens compared to conventional ones. Functional analyses including Gene Ontology (GO) and STRING network analyses revealed that proteins upregulated in organic chickens were predominantly involved in oxygen transport, oxygen binding, and muscle structural organization, indicating enhanced oxygen metabolism and muscle development consistent with improved animal welfare. Conversely, proteins related to ribosomal function and RNA binding were enriched in conventional chickens, suggesting stress-related alterations in protein synthesis. KEGG pathway analysis showed significant enrichment of carbon metabolism, amino acid biosynthesis, nitrogen metabolism, and the tricarboxylic acid (TCA) cycle pathways in organic chickens, while glycolysis, gluconeogenesis, and ribosomal pathways were downregulated. Key differentially expressed proteins identified as potential biomarkers distinguishing organic from conventional meat include downregulated PGM1, AMPD1, LDHA, ENO3, and PKLR, and upregulated COL1A1, COL1A2, TTN, TPM2, CA3, MB, HSPB1, ACO2, ACAA2, and TF. These proteins are involved in muscle structure and energy metabolism and may serve as indicators of meat quality linked to stress and welfare conditions. Overall, this proteomic analysis provides novel insights into how stress modulates the muscle proteome in broiler chickens and supports the adoption of welfare-focused organic poultry production practices to improve meat quality. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-28_16:16:06.453.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD066589 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ivanka Krasteva |
| SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-25 05:06:05 | ID requested | |
| ⏵ 1 | 2025-12-28 16:16:07 | announced |
Publication List
| 10.6019/PXD066589; |
| Luciani M, Schirone M, Krasteva I, Pancrazio CD, Sacchini F, Maggetti M, Perletta F, D'Alterio N, Paparella A, Vulpiani MP, Iannetti L, Muscle proteomics reveals biomarkers of stress and meat quality in organic versus conventional broiler chickens. Poult Sci, 105(1):106164(2026) [pubmed] |
| 10.1016/j.psj.2025.106164; |
Keyword List
| submitter keyword: label-free quantification |
| stress biomarkers |
| chicken welfare |
| meat traits |
| proteomics |
Contact List
| Mirella Luciani | |
|---|---|
| contact affiliation | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" |
| contact email | m.luciani@izs.it |
| lab head | |
| Ivanka Krasteva | |
| contact affiliation | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" |
| contact email | i.krasteva@izs.it |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD066589 |
| PRIDE project URI |
Repository Record List
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