PXD066570

PXD066570 is an original dataset announced via ProteomeXchange.

Title	Molecular characterization of the group A Streptococcus virulence-regulatory system FasBCAX
Description	By regulating the assortment and abundance of its virulence factors at different anatomic sites, the group A Streptococcus (GAS) can cause a range of human diseases. The Fas regulatory system is encoded by a four gene locus, fasBCAX, with fasX encoding the FasX small regulatory RNA effector molecule. FasX post-transcriptionally regulates target mRNAs through well-characterized mechanisms. Less characterized are the layers of regulation that occur upstream of FasX activity, such as how the products of the fasBCA genes enhance FasX abundance 100-fold. Here, we present data consistent with FasBCA forming a three-component regulatory system, with FasBC being sensor kinase-like proteins that, upon recognizing one or more signals, heterodimerize and phosphorylate FasA, with phosphorylated FasA binding to the fasX promoter and inducing transcription. We identified key amino acids involved in phosphate flow, including H246 of FasC and D60 of FasA, and demonstrated that certain domains (e.g., the kinase domain of FasC) are dispensable for activity. Additionally, we show that a proteinaceous factor within human plasma activates the Fas system. This work represents the first molecular analysis of the Fas proteins which, by modulating FasX levels, play a critical role in the ability of GAS to coordinately regulate virulence factor production.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-20_10:18:22.121.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sushila Baral
SpeciesList	scientific name: Streptococcus pyogenes M1 GAS; NCBI TaxID: NEWT:160490;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-24 15:55:23	ID requested
⏵ 1	2025-10-20 10:18:22	announced

10.1111/MMI.70029;

submitter keyword: group A Streptococcus (GAS), plasma, Fas regulatory system, heterodimerize, FasX small regulatory RNA

Paul Sumby
contact affiliation	Department of Microbiology & Immunology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA.
contact email	psumby@med.unr.edu
lab head
Sushila Baral
contact affiliation	University of Nevada Reno
contact email	sushilab@unr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066570
     1. Label: PRIDE project
     2. Name: Molecular characterization of the group A Streptococcus virulence-regulatory system FasBCAX