PXD066568 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lipid-specific metabolic labelling reveals site-specific heterogeneity in long-chain S-acylation |
| Description | Long-chain S-acylation is a post-translational modification that regulates key cellular processes, including signal transduction and metabolic regulation. However, the dynamic nature, and site- and lipid-specific patterns of long-chain protein acylation remain poorly understood. Site- and lipid-specific metabolic labelling with various ω-alkynyl fatty acids uncovered the site-specific heterogeneity of long-chain protein S-acylation. Cells use various fatty acids for long-chain S-acylation, including C16:0, C18:0, and C18:1 on cysteines, while N-myristoylation preferentially incorporates C14:0 on N-terminal glycine residues. Our results demonstrate that long-chain S-acylation sites can exhibit both lipid heterogeneity and specificity and reveal that both enzymatic specificity and metabolic context can influence fatty acid incorporation. Exploration of the dy-namic protein long-chain S-acylation uncovered that acyl-protein thioesterases targeted by Pal-mostatin B regulate long-chain S-acylation involving various lipids, including C16:0, C18:0, and C18:1. Our approach represents a significant advancement in lipid metabolic labelling methodol-ogies, offering enhanced efficiency and sensitivity for studying S-acylation dynamics with lipid- and site-specificity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-15 |
| AnnouncementXML | Submission_2026-07-15_06:06:56.670.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Anneroos Nederstigt |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | N-ethylmaleimide derivatized cysteine; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-24 15:28:39 | ID requested | |
| 1 | 2026-06-30 09:13:39 | announced | |
| ⏵ 2 | 2026-07-15 06:06:57 | announced | 2026-07-15: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: liquid chromatography, tandem mass spectrom-etry, lipid metabolic labelling,Long-chain S-acylation |
Contact List
| Marc Pieter |
|---|
| contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht 3584 CH The Netherlands Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH, The Netherlands |
| contact email | m.p.baggelaar@uu.nl |
| lab head | |
| Anneroos Nederstigt |
|---|
| contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht |
| contact email | a.e.nederstigt@uu.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066568
- Label: PRIDE project
- Name: Lipid-specific metabolic labelling reveals site-specific heterogeneity in long-chain S-acylation
